LOCUS       BBa_J11031              1558 bp    DNA     linear       09-May-2024
DEFINITION  Bacterial Whiteboard by Hannah (w/ Minimal Available Parts)
ACCESSION   BBa_J11031
VERSION     BBa_J11031.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..428
                     /label=lacZ alpha
     misc_feature    161..161
                     /label=T
     misc_feature    167..167
                     /label=A
     misc_feature    188..188
                     /label=C
     misc_feature    158..158
                     /label=C
     misc_feature    191..191
                     /label=G
     promoter        106..115
                     /label=T3 promoter
     promoter        253..271
                     /label=T7 promoter
     misc_feature    279..428
                     /label=lacZ gene fragment
     misc_feature    81..95
                     /label=lacZ gene fragment
     misc_feature    81..433
                     /label=BBa_E0033
     stem_loop       453..496
                     /label=stem_loop
     misc_feature    442..521
                     /label=BBa_B0010
     terminator      442..521
                     /label=BBa_B0010
     stem_loop       537..556
                     /label=T7 TE
     misc_feature    563..563
                     /label=stop
     polyA_site      557..570
                     /label=polya
     misc_feature    530..570
                     /label=BBa_B0012
     terminator      530..570
                     /label=BBa_B0012
     terminator      442..570
                     /label=BBa_B0015
     misc_feature    63..570
                     /label=BBa_E0433
     misc_feature    581..597
                     /label=lac O1
     promoter        598..603
                     /label=-35
     misc_feature    604..620
                     /label=lac O1
     promoter        621..626
                     /label=-10
     misc_feature    579..632
                     /label=BBa_R0011
     promoter        579..632
                     /label=BBa_R0011
     misc_feature    646..649
                     /label=conserved
     RBS             642..653
                     /label=BBa_B0034
     CDS             660..1421
                     /label=CFP (LVA)
     misc_feature    1378..1415
                     /label=SsrA
     misc_feature    1416..1421
                     /label=2
     misc_feature    728..728
                     /label=A
     misc_feature    660..1421
                     /label=BBa_E0022
     misc_feature    660..1421
                     /label=BBa_E0022
     stem_loop       1441..1484
                     /label=stem_loop
     misc_feature    1430..1509
                     /label=BBa_B0010
     terminator      1430..1509
                     /label=BBa_B0010
     stem_loop       1525..1544
                     /label=T7 TE
     misc_feature    1551..1551
                     /label=stop
     polyA_site      1545..1558
                     /label=polya
     misc_feature    1518..1558
                     /label=BBa_B0012
     terminator      1518..1558
                     /label=BBa_B0012
     misc_feature    642..1558
                     /label=BBa_E0422
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaccatga ttacgccaag cgcgcaatta accctcacta
      121 aagggaacaa aagctggagc tccaccgcgg tggcggcagc actagagcta gtggatcccc
      181 cgggctgtag aaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
      241 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      301 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      361 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      421 gcctgaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      481 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      541 caccttcggg tgggcctttc tgcgtttata tactagagaa ttgtgagcgg ataacaattg
      601 acattgtgag cggataacaa gatactgagc acatactaga gaaagaggag aaatactaga
      661 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      721 gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      781 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      841 tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      901 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      961 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1021 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1081 agctggagta caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg
     1141 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1201 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1261 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1321 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaggc
     1381 ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc caggcatcaa
     1441 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1501 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//