##gff-version 3
##sequence-region BBa_J11031 1 1558
BBa_J11031	.	promoter	579	632	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J11031	.	promoter	598	603	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J11031	.	promoter	621	626	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J11031	.	operator	581	597	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J11031	.	operator	604	620	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J11031	.	engineered_region	579	632	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J11031	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J11031	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J11031	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J11031	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J11031	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J11031	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J11031	.	engineered_region	63	570	.	+	0	ID=BBa_E0433;Name=BBa_E0433
BBa_J11031	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0433
BBa_J11031	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J11031	.	terminator	442	570	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0433
BBa_J11031	.	terminator	442	521	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J11031	.	stem_loop	453	496	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11031	.	engineered_region	442	521	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11031	.	terminator	530	570	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J11031	.	engineered_region	530	570	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11031	.	stop_codon	563	563	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11031	.	stem_loop	537	556	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11031	.	polyA_site	557	570	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11031	.	engineered_region	81	433	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_E0433
BBa_J11031	.	sequence_alteration	191	191	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_J11031	.	sequence_alteration	158	158	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_J11031	.	sequence_alteration	188	188	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_J11031	.	sequence_feature	81	95	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_J11031	.	sequence_feature	279	428	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_J11031	.	sequence_alteration	167	167	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_J11031	.	promoter	253	271	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_J11031	.	CDS	81	428	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_J11031	.	sequence_alteration	161	161	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_J11031	.	promoter	106	115	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_J11031	.	engineered_region	642	1558	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_J11031	.	engineered_region	660	1421	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_J11031	.	CDS	660	1421	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J11031	.	tag	1378	1415	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J11031	.	engineered_region	660	1421	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J11031	.	point_mutation	728	728	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J11031	.	stop_codon	1416	1421	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J11031	.	terminator	1430	1509	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_J11031	.	stem_loop	1441	1484	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11031	.	engineered_region	1430	1509	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11031	.	terminator	1518	1558	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_J11031	.	engineered_region	1518	1558	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11031	.	stop_codon	1551	1551	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11031	.	stem_loop	1525	1544	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11031	.	polyA_site	1545	1558	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11031	.	ribosome_entry_site	642	653	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_J11031	.	conserved_region	646	649	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J11031
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggca
gcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcc
cggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaa
ccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcacc
gatcgcccttcccaacagttgcgcagcctgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcac
atactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcg
agctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgac
cctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcag
tgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagc
gcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccg
catcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccac
aacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggca
gcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacct
gagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgcc
gggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata