##gff-version 3
##sequence-region BBa_J11051 1 570
BBa_J11051	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J11051	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J11051	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J11051	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J11051	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J11051	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J11051	.	engineered_region	63	570	.	+	0	ID=BBa_E0433;Name=BBa_E0433
BBa_J11051	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0433
BBa_J11051	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J11051	.	terminator	442	570	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0433
BBa_J11051	.	terminator	530	570	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J11051	.	stem_loop	537	556	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J11051	.	polyA_site	557	570	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J11051	.	stop_codon	563	563	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J11051	.	engineered_region	530	570	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J11051	.	terminator	442	521	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J11051	.	stem_loop	453	496	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J11051	.	engineered_region	442	521	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J11051	.	engineered_region	81	433	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_E0433
BBa_J11051	.	promoter	253	271	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_J11051	.	sequence_alteration	158	158	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_J11051	.	promoter	106	115	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_J11051	.	sequence_alteration	167	167	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_J11051	.	sequence_alteration	161	161	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_J11051	.	CDS	81	428	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_J11051	.	sequence_alteration	188	188	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_J11051	.	sequence_alteration	191	191	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_J11051	.	sequence_feature	279	428	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_J11051	.	sequence_feature	81	95	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
>BBa_J11051
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggca
gcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcc
cggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaa
ccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcacc
gatcgcccttcccaacagttgcgcagcctgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata