LOCUS       BBa_J119000             3124 bp    DNA     linear       22-Dec-2024
DEFINITION  pT7-RBS-lacZ
ACCESSION   BBa_J119000
VERSION     BBa_J119000.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    3073..3073
                     /label=C->T
     CDS             50..3124
                     /label=lacZ
     misc_feature    50..52
                     /label=START
     misc_feature    3122..3124
                     /label=STOP
     CDS             50..3124
                     /label=BBa_I732005
     misc_feature    32..3124
                     /label=BBa_I732017
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgaccatgat
       61 tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      121 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      181 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt
      241 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac
      301 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt
      361 gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta
      421 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt
      481 tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca
      541 ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg
      601 cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg
      661 gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag
      721 cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga
      781 agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg
      841 tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg
      901 tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc
      961 cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat
     1021 tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct
     1081 gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca
     1141 tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa
     1201 ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga
     1261 ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat
     1321 gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat
     1381 ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg
     1441 ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc
     1501 ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta
     1561 cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg
     1621 gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg
     1681 taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca
     1741 gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa
     1801 cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat
     1861 gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca
     1921 gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct
     1981 gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct
     2041 ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc
     2101 tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc
     2161 gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc
     2221 ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag
     2281 cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg
     2341 ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca
     2401 gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc
     2461 taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt
     2521 gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca
     2581 gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca
     2641 aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg
     2701 cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca
     2761 agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc
     2821 agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga
     2881 attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag
     2941 tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg
     3001 gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt
     3061 atcggcggaa tttcagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa
     3121 ataa
//