BBa_J119002 1 BBa_J119002 BsaI-GFP2-BsmBI 2011-07-20T11:00:00Z 2015-08-31T04:08:26Z PCR Amplification of GFP gene template split at the sequence ACCC at 351 nt from start. This is the second half of the GFP gene split for use in solving HPP (Hamiltonian Path Problems). It is the necessary complement to GFP-1 part name: BBa_J119001 It will function as a reporter if both genes are transcribed in proper order after re-assembly in E Coli. It is also intended to be mixed with other gene halves to attempt a multi-node HPP problem that will show correct completion if all reporters are present (resistance, fluorescence, etc.). All groupings must have both halves of a specific reporter gene to function properly. Here is the design of the forward and reverse sequences minus the entire gene sequence. GFP_2_For (36mer) GCAT GAATTC GGTCTC A ACCC TTGTTAATAGAATCG 4 EcoRI BsaI a 1234 15mer of GFP_2 GFP_2_Rev (158mer) ATGC CTGCAG CGTCTC A CCAC GTGCTCAGTATCTCTATCACTGATAGGGATGTCAATCTCTATCACTGATAGGGA TTGC 4 PstI BsmBI a 1234 pTet Reverse Complement 4 (1/2 edge R.C.) false false _613_ 0 10385 9 Not in stock false There is a rogue BsaI site at position 304 causing 457 bp to split into 304 bp and 153 bp. It can be removed or altered to lessen the occurrence of additional cutting at that site. false Caleb J. Carr annotation2167071 1 EcoRI range2167071 1 1 6 annotation2167072 1 pTet R0040 range2167072 1 387 440 BBa_J119002_sequence 1 gaattcggtctcaacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataagcaatccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtggtgagacgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z