BBa_J119002
1
BBa_J119002
BsaI-GFP2-BsmBI
2011-07-20T11:00:00Z
2015-08-31T04:08:26Z
PCR Amplification of GFP gene template split at the sequence ACCC at 351 nt from start.
This is the second half of the GFP gene split for use in solving HPP (Hamiltonian Path Problems). It is the necessary complement to GFP-1 part name: BBa_J119001
It will function as a reporter if both genes are transcribed in proper order after re-assembly in E Coli.
It is also intended to be mixed with other gene halves to attempt a multi-node HPP problem that will show correct completion if all reporters are present (resistance, fluorescence, etc.). All groupings must have both halves of a specific reporter gene to function properly.
Here is the design of the forward and reverse sequences minus the entire gene sequence.
GFP_2_For (36mer)
GCAT GAATTC GGTCTC A ACCC TTGTTAATAGAATCG
4 EcoRI BsaI a 1234 15mer of GFP_2
GFP_2_Rev (158mer)
ATGC CTGCAG CGTCTC A CCAC GTGCTCAGTATCTCTATCACTGATAGGGATGTCAATCTCTATCACTGATAGGGA TTGC
4 PstI BsmBI a 1234 pTet Reverse Complement 4
(1/2 edge R.C.)
false
false
_613_
0
10385
9
Not in stock
false
There is a rogue BsaI site at position 304 causing 457 bp to split into 304 bp and 153 bp. It can be removed or altered to lessen the occurrence of additional cutting at that site.
false
Caleb J. Carr
annotation2167071
1
EcoRI
range2167071
1
1
6
annotation2167072
1
pTet R0040
range2167072
1
387
440
BBa_J119002_sequence
1
gaattcggtctcaacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataagcaatccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtggtgagacgctgcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z