BBa_J119003 1 BBa_J119003 BsmBI-Rbs-LacZ1-BsaI 2011-07-20T11:00:00Z 2015-08-31T04:08:26Z PCR amplification of LacZ. First half of the LacZ Gene (LacZ-1), split for use in HPP (Hamiltonian Path Problems). It is the necessary complement half of BBa_J119004, without the other, neither will function. They are intended to be re-assembled via transformation in E Coli Jm109. It functions as a reporter gene when properly assembled with LacZ-2 by producing blue coloring (non-fluorescing) when X-Gal is present. Can be used in conjunction with other split genes but must have the complement to report properly. Beta-Gal_1_For (58mer) 5??? GCAT GAATTC CGTCTC A GTGG CA AAAGAGGAGAAA CGTACGAC ATGACCATGATTACG 3??? (4nt , EcoRI, BsmBI , a , Halfedge1234, nn, RBS 0034 , 8 , 15mer of Beta-Gal) Beta-Gal_1_Rev (36mer) 5???ATGC CTGCAG GGTCTC A CAGG TAGCGAAAGCCATTT 3???) (4nt, PstI , BsaI , a , Gene1234RC , 15mer of Beta-Gal reverse false false _613_ 0 10385 9 Not in stock false This is NOT a BioBrick part. It is only functional if properly re-assembled with J11904. Can be used in combination with other split-genes to solve HPP (Hamiltonian Path Problems). false Caleb J. Carr BBa_J119003_sequence 1 gaattccgtctcagtggcaaaagaggagaaacgtacgacatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctgtgagaccctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z