BBa_J119004 1 BBa_J119004 BsaI-LacZ2-BsmbI 2011-07-20T11:00:00Z 2015-08-31T04:08:26Z PCR amplification of the LacZ template. Second half of the LacZ Gene (LacZ-2), split for use in HPP (Hamiltonian Path Problems). It is the necessary complement half of BBa_J119003, without the other, neither will function. They are intended to be re-assembled via transformation in E Coli JM109. It functions as a reporter gene when properly assembled with LacZ-1 by producing blue coloring (non-fluorescing) when X-Gal is present. Can be used in conjunction with other split genes but must have the complement to report properly. Beta-Gal_2_For (36mer) 5??? GCAT GAATTC GGTCTC A CCTG GAGAGGCGCGCCCGC 3??? (4, EcoRI , BsaI , a , Gene1234 , 15mer of Beta-Gal) Beta-Gal_2_Rev (96mer) 5???ATGC CTGCAG CGTCTC A CCAC CCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCAACG TTGC TTATTTTTGACACCA 3??? (4 , PstI , BsmBI , a , Halfedge1234RC , pLacI rc , 4mer , 15mer of Beta-Gal Rev) false false _613_ 0 10385 9 Not in stock false Update false Caleb J. Carr BBa_J119004_sequence 1 gaattcggtctcacctggagaggcgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccggacacatcagcgcctggcagcagtggcgtctggctgaaaacctcagcgtgacactccccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtggattggcgataaaaaacaactgctgacgccgctgcgcgatcagttcgcccgtgcaccgctggataacgacattggcgtaagtgaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgcacggcagatacacttgctgatgcggtgctgattacggccgctcacgcgtggcagcatcaggggaaaaccttatttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcgatacaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtataccccgtacgtctccccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcgacttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaatttcagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataagcaacgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccgggtggtgagacgctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z