LOCUS       BBa_J119009             2805 bp    DNA     linear       09-Dec-2025
DEFINITION  pLacI-RBS-T7RNAP with sites for oligo insertion
ACCESSION   BBa_J119009
VERSION     BBa_J119009.1  
FEATURES             Location/Qualifiers
     promoter        1..56
                     /label=pLacI
     misc_feature    57..62
                     /label=BsmBI
     RBS             68..82
                     /label=B0030
     misc_feature    89..91
                     /label=Start RNAP
     misc_feature    99..104
                     /label=BsaI
     misc_feature    107..114
                     /label=AsisI
     misc_feature    117..124
                     /label=Asc1
     misc_feature    126..131
                     /label=BsaI
     misc_feature    137..142
                     /label=BbsI
     misc_feature    2800..2805
                     /label=BsmBI
     misc_feature    2789..2791
                     /label=Stop RNAP
     CDS             89..2788
                     /label=T7 RNA Polymerase
ORIGIN
        1 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggcgtc
       61 tcggcgaatt aaagaggaga aagtaggcat gaacacgaga gaccacgcga tcgcgaggcg
      121 cgccaggtct ctgctggaag acgtcacgat taacatcgct aagaacgact tctctgacat
      181 cgaactggct gctatcccgt tcaacactct agctgaccat tacggtgagc gtttagctcg
      241 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat
      301 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat
      361 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc
      421 taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt
      481 agcgtacatc accattaaga ccactctagc ttgcctaacc agtgctgaca atacaaccgt
      541 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat
      601 ccgtgacctt gaagctaagc acttcaagaa aaacgtcgag gaacaactca acaagcgcgt
      661 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg
      721 tctactcggt ggcgaggcgt ggtcctcgtg gcataaggag gactctattc atgtaggagt
      781 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc
      841 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat
      901 cgcaacccgt gcaggggcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc
      961 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct
     1021 agcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaggacg tttacatgcc
     1081 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt
     1141 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc
     1201 gattgagcgt gaagaactcc cgatgaaacc ggaggacatc gacatgaatc ctgaggctct
     1261 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg
     1321 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat
     1381 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc
     1441 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa
     1501 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc
     1561 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa
     1621 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt
     1681 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct
     1741 ggcgtttgac gggtcttgct ccggcatcca gcacttctcc gcgatgctcc gagatgaggt
     1801 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt
     1861 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt
     1921 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa
     1981 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt
     2041 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac
     2101 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc
     2161 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga
     2221 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa
     2281 aactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc
     2341 tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca
     2401 gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca
     2461 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac
     2521 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt
     2581 cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga
     2641 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca
     2701 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga
     2761 catcttagag tcggacttcg cgttcgcgta ataacgcatg agacg
//