BBa_J119011
1
BBa_J119011
BsaI-GFP2 (BsaI removed)-BsmBI
2011-08-02T11:00:00Z
2015-08-31T04:08:26Z
PCR Amplification of existing part: GFP (E0040)split 351 bp from the beginning of the gene at with the sticky word ACCC
This is GFP-2A, the second half of the GFP Gene Template to be used in conjunction with RFP-1 (J119005) to act as a node for solving Hamiltonian Path Problems (HPP). When successfully re-assembled will report with a red fluorescence that may be visible under normal light conditions. Can be used with other split genes to solve more complex HPP problems.
false
false
_613_
0
10447
9
Not in stock
false
This is NOT a BioBrick compatible part. It was designed to solve Hamiltonian Path Problems using multiple genes.
There is a rogue BsaI site at position 304 causing 457 bp to split into 304 bp and 153 bp. It can be removed or altered to lessen the occurrence of additional cutting at that site.
false
Zachary Caton
annotation2124260
1
Sticky Word: ACCC
range2124260
1
14
17
annotation2124267
1
PstI
range2124267
1
452
457
annotation2124261
1
BsaI
range2124261
1
7
13
annotation2124266
1
Sticky Word: GTGG
range2124266
1
441
444
annotation2124262
1
EcoRI
range2124262
1
2
6
annotation2124263
1
Rogue BsaI site
range2124263
1
305
311
annotation2124265
1
BsmBI
range2124265
1
445
451
annotation2124264
1
Sticky Word: GCAA
range2124264
1
383
386
BBa_J119011_sequence
1
gaattcggtctcaacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataagcaatccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtggtgagacgctgcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z