LOCUS BBa_J119012 5937 bp DNA linear 26-Nov-2024 DEFINITION pT7-RBS-LacZ-pLac-RBS-T7RNAP ACCESSION BBa_J119012 VERSION BBa_J119012.1 FEATURES Location/Qualifiers misc_feature 18..18 /label=Transcription Start Site misc_feature 1..11 /label=Polymerase Binding Region misc_feature 12..23 /label=Initiation Region promoter 1..23 /label=BBa_R0085 misc_feature 36..39 /label=conserved RBS 32..43 /label=BBa_B0034 misc_feature 3073..3073 /label=C->T CDS 50..3124 /label=lacZ misc_feature 50..52 /label=START misc_feature 3122..3124 /label=STOP CDS 50..3124 /label=BBa_I732005 misc_feature 32..3124 /label=BBa_I732017 misc_feature 1..3124 /label=BBa_J119000 promoter 3133..3188 /label=pLacI misc_feature 3189..3194 /label=BsmBI RBS 3200..3214 /label=B0030 misc_feature 3221..3223 /label=Start RNAP misc_feature 3231..3236 /label=BsaI misc_feature 3239..3246 /label=AsisI misc_feature 3249..3256 /label=Asc1 misc_feature 3258..3263 /label=BsaI misc_feature 3269..3274 /label=BbsI misc_feature 5932..5937 /label=BsmBI misc_feature 5921..5923 /label=Stop RNAP CDS 3221..5920 /label=T7 RNA Polymerase CDS 3133..5937 /label=BBa_J119009 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgaccatgat 61 tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 121 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 181 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt 241 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac 301 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt 361 gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta 421 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt 481 tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca 541 ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg 601 cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg 661 gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag 721 cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga 781 agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg 841 tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg 901 tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc 961 cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat 1021 tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct 1081 gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca 1141 tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa 1201 ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga 1261 ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat 1321 gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat 1381 ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg 1441 ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc 1501 ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta 1561 cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg 1621 gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg 1681 taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca 1741 gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa 1801 cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat 1861 gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca 1921 gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct 1981 gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct 2041 ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc 2101 tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc 2161 gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc 2221 ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag 2281 cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg 2341 ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca 2401 gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc 2461 taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt 2521 gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca 2581 gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca 2641 aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg 2701 cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca 2761 agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc 2821 agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga 2881 attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag 2941 tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg 3001 gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt 3061 atcggcggaa tttcagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa 3121 ataatactag agcgttgaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata 3181 gcgcccggcg tctcggcgaa ttaaagagga gaaagtaggc atgaacacga gagaccacgc 3241 gatcgcgagg cgcgccaggt ctctgctgga agacgtcacg attaacatcg ctaagaacga 3301 cttctctgac atcgaactgg ctgctatccc gttcaacact ctagctgacc attacggtga 3361 gcgtttagct cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg 3421 cttccgcaag atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc 3481 caagcctctc atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga 3541 ggaagtgaaa gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa 3601 gccggaagcc gtagcgtaca tcaccattaa gaccactcta gcttgcctaa ccagtgctga 3661 caatacaacc gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg 3721 cttcggtcgt atccgtgacc ttgaagctaa gcacttcaag aaaaacgtcg aggaacaact 3781 caacaagcgc gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat 3841 gctctctaag ggtctactcg gtggcgaggc gtggtcctcg tggcataagg aggactctat 3901 tcatgtagga gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca 3961 ccgccaaaat gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata 4021 cgctgaggct atcgcaaccc gtgcaggggc gctggctggc atctctccga tgttccaacc 4081 ttgcgtagtt cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg 4141 tcgtcgtcct ctagcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgagga 4201 cgtttacatg cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat 4261 caacaagaaa gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga 4321 ggacatccct gcgattgagc gtgaagaact cccgatgaaa ccggaggaca tcgacatgaa 4381 tcctgaggct ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc 4441 tcgcaagtct cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa 4501 ccataaggcc atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc 4561 aatgttcaac ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa 4621 accaatcggt aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt 4681 cgataaggtt ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat 4741 ggcttgcgct aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg 4801 cttccttgcg ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg 4861 ctcccttccg ctggcgtttg acgggtcttg ctccggcatc cagcacttct ccgcgatgct 4921 ccgagatgag gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat 4981 ctacgggatt gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac 5041 cgataacgaa gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa 5101 gctgggcact aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac 5161 taagcgttca gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt 5221 gctggaagat accattcagc cagctattga ttccggcaag ggtctgatgt tcactcagcc 5281 gaatcaggct gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt 5341 agctgcggtt gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt 5401 caaagataag aaaactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc 5461 tgatggtttc cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat 5521 gttcctcggt cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga 5581 tgcacacaaa caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca 5641 ccttcgtaag actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat 5701 tcacgactcc ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga 5761 aactatggtt gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc 5821 tgaccagttg cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt 5881 gaacctccgt gacatcttag agtcggactt cgcgttcgcg taataacgca tgagacg //