##gff-version 3
##sequence-region BBa_J119012 1 5937
BBa_J119012	.	CDS	3133	5937	.	+	0	ID=BBa_J119009;Name=BBa_J119009
BBa_J119012	.	sequence_feature	3239	3246	.	+	0	ID=annotation2169627;Name=AsisI;Parent=BBa_J119009
BBa_J119012	.	start_codon	3221	3223	.	+	0	ID=annotation2169625;Name=Start RNAP;Parent=BBa_J119009
BBa_J119012	.	promoter	3133	3188	.	+	0	ID=annotation2169622;Name=pLacI;Parent=BBa_J119009
BBa_J119012	.	sequence_feature	3189	3194	.	+	0	ID=annotation2169623;Name=BsmBI;Parent=BBa_J119009
BBa_J119012	.	sequence_feature	3258	3263	.	+	0	ID=annotation2169629;Name=BsaI;Parent=BBa_J119009
BBa_J119012	.	stop_codon	5921	5923	.	+	0	ID=annotation2169632;Name=Stop RNAP;Parent=BBa_J119009
BBa_J119012	.	sequence_feature	3249	3256	.	+	0	ID=annotation2169628;Name=Asc1;Parent=BBa_J119009
BBa_J119012	.	sequence_feature	5932	5937	.	+	0	ID=annotation2169631;Name=BsmBI;Parent=BBa_J119009
BBa_J119012	.	sequence_feature	3231	3236	.	+	0	ID=annotation2169626;Name=BsaI;Parent=BBa_J119009
BBa_J119012	.	sequence_feature	3269	3274	.	+	0	ID=annotation2169630;Name=BbsI;Parent=BBa_J119009
BBa_J119012	.	CDS	3221	5920	.	+	0	ID=annotation2169633;Name=T7 RNA Polymerase;Parent=BBa_J119009
BBa_J119012	.	ribosome_entry_site	3200	3214	.	+	0	ID=annotation2169624;Name=B0030;Parent=BBa_J119009
BBa_J119012	.	engineered_region	1	3124	.	+	0	ID=BBa_J119000;Name=BBa_J119000
BBa_J119012	.	engineered_region	32	3124	.	+	0	ID=BBa_I732017;Name=BBa_I732017;Parent=BBa_J119000
BBa_J119012	.	CDS	50	3124	.	+	0	ID=BBa_I732005;Name=BBa_I732005;Parent=BBa_I732017
BBa_J119012	.	CDS	50	3124	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_J119012	.	point_mutation	3073	3073	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_J119012	.	start_codon	50	52	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_J119012	.	stop_codon	3122	3124	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_J119012	.	ribosome_entry_site	32	43	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732017
BBa_J119012	.	conserved_region	36	39	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J119012	.	promoter	1	23	.	+	0	ID=BBa_R0085;Name=BBa_R0085;Parent=BBa_J119000
BBa_J119012	.	sequence_feature	12	23	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_J119012	.	non_covalent_binding_site	1	11	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_J119012	.	sequence_feature	18	18	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
>BBa_J119012
taatacgactcactatagggagatactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtc
gttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagct
ggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctg
gtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctca
aactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttc
ccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaat
tatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttg
ccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacg
gcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactac
acaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatg
tgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgc
ctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaa
actgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagca
gaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattc
gaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgct
gatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgc
tacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgc
gctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtc
gctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccg
gtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagacc
agcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttg
cgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgttta
cagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacg
gcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatcc
agcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaa
tacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcggtgaag
tgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgccgggcaact
ctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctggcagcagtgg
cgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagcgaaatggatt
tttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtggattggcgataa
aaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaagtgaagcgacc
cgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttgttgcagtgca
cggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaaccttatttatcag
ccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcgatacaccgcat
ccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattagggccgcaagaaa
actatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtataccccgtacgtctt
cccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcgacttccagttc
aacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaaggcacatggc
tgaatatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaatttcagctgag
cgccggtcgctaccattaccagttggtctggtgtcaaaaataatactagagcgttgacaccatcgaatggcgcaaaacc
tttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacg
cgatcgcgaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaact
ggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgag
tcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctg
ccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaa
gcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagacc
actctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacg
aggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgt
agggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcg
tggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatgg
ttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggc
tatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggact
ggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactga
tgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaa
gaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaa
gaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgt
accgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaacca
taaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaac
gatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacg
gtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcat
ggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgcttt
gagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctccggca
tccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcagga
catctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagtt
accgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctgg
cttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtca
acaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgct
ggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagt
ctgctgctaagctgctggctgctgaggtcaaagataagaaaactggagagattcttcgcaagcgttgcgctgtgcattg
ggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggt
cagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctc
ctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatc
ttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatg
gttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattgg
acaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaata
acgcatgagacg