BBa_J119012
type: ComponentDefinition
persistent identity: BBa J119012
display id: BBa_J119012
version: 1
was generated by: iGEM to SBOL conversion
description: pT7-RBS-LacZ-pLac-RBS-T7RNAP
created: 2011-08-15T11:00:00Z
modified: 2015-08-31T04:08:26Z
mutable provenance: This part came from two previous parts J119000 and J119009.
owned by: james
owned by: myers
mutable description: This part consists of both T7RNAP and the T7RNAP promoter. The gene produces a blue color when on a plate that contains X-gal. The gene produces T7RNAP which attaches to the T7RNAP promoter this produces LacZ and a blue color.
discontinued: false
dominant: false
experience: experience/None
group u list: _613_
m user id: 0
owner id: 10415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Michel Conn
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J119000
component: BBa_J119009
sequence annotation: BBa_J119000
sequence annotation: BBa_J119009
sequence: https://synbiohub.org/public/igem/BBa_J119012_sequence/1

BBa_J119000
type: Component
persistent identity: component2220730
display id: component2220730
version: 1
top level: BBa_J119012
definition: pT7-rbs-la
access: public

BBa_J119009
type: Component
persistent identity: component2220743
display id: component2220743
version: 1
top level: BBa_J119012
definition: BBa_J119009
access: public

BBa_J119000
type: SequenceAnnotation
persistent identity: annotation2220730
display id: annotation2220730
version: 1
top level: BBa_J119012
location: https://synbiohub.org/public/igem/BBa_J119012/annotation2220730/range2220730/1
component: BBa_J119000

https://synbiohub.org/public/igem/BBa_J119012/annotation2220730/range2220730/1
type: Range
persistent identity: range2220730
display id: range2220730
version: 1
top level: BBa_J119012
direction: direction/reverse
start: 1
end: 3124
orientation: inline

BBa_J119009
type: SequenceAnnotation
persistent identity: annotation2220743
display id: annotation2220743
version: 1
top level: BBa_J119012
location: https://synbiohub.org/public/igem/BBa_J119012/annotation2220743/range2220743/1
component: BBa_J119009

https://synbiohub.org/public/igem/BBa_J119012/annotation2220743/range2220743/1
type: Range
persistent identity: range2220743
display id: range2220743
version: 1
top level: BBa_J119012
direction: direction/reverse
start: 3133
end: 5937
orientation: inline

pT7-rbs-la
type: ComponentDefinition
persistent identity: BBa J119000
display id: BBa_J119000
version: 1
was generated by: iGEM to SBOL conversion
description: pT7-RBS-lacZ
created: 2011-07-19T11:00:00Z
modified: 2015-08-31T04:08:26Z
mutable provenance: We used parts I732017 and R0085 to construct the pT7-rbs-lacZ reporter.
owned by: james
owned by: myers
mutable description: When inserted, cells will have a functioning beta-galactosidase gene. If placed onto a plate with X-Gal, cells with this functioning part will split apart the X-gal and colonies will appear blue.
discontinued: false
dominant: false
experience: experience/None
group u list: _613_
m user id: 0
owner id: 10379
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Cody Barta
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0085
component: BBa_I732017
sequence annotation: BBa_R0085
sequence annotation: BBa_I732017
sequence: https://synbiohub.org/public/igem/BBa_J119000_sequence/1

BBa_R0085
type: Component
persistent identity: component2123832
display id: component2123832
version: 1
top level: pT7-rbs-la
definition: BBa_R0085
access: public

BBa_I732017
type: Component
persistent identity: component2123840
display id: component2123840
version: 1
top level: pT7-rbs-la
definition: BBa_I732017
access: public

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation2123832
display id: annotation2123832
version: 1
top level: pT7-rbs-la
location: https://synbiohub.org/public/igem/BBa_J119000/annotation2123832/range2123832/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_J119000/annotation2123832/range2123832/1
type: Range
persistent identity: range2123832
display id: range2123832
version: 1
top level: pT7-rbs-la
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_I732017
type: SequenceAnnotation
persistent identity: annotation2123840
display id: annotation2123840
version: 1
top level: pT7-rbs-la
location: https://synbiohub.org/public/igem/BBa_J119000/annotation2123840/range2123840/1
component: BBa_I732017

https://synbiohub.org/public/igem/BBa_J119000/annotation2123840/range2123840/1
type: Range
persistent identity: range2123840
display id: range2123840
version: 1
top level: pT7-rbs-la
direction: direction/reverse
start: 32
end: 3124
orientation: inline

BBa_J119009
type: ComponentDefinition
persistent identity: BBa J119009
display id: BBa_J119009
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI-RBS-T7RNAP with sites for oligo insertion
created: 2011-07-26T11:00:00Z
modified: 2015-08-31T04:08:26Z
mutable provenance: GeneArt
owned by: james
owned by: myers
mutable description: J23100 promoter and B0030 RBS upstream of T7 RNAP gene. The following restriction sites do not occur in the promoter-RBS-T7 RNAP sequence. Some of them did not occur in the original sequence and others were removed while conserving the amino acid sequence. Both the forward and reverse complement were checked for type IIs enzyme sites. EcoRI, XbaI, SpeI, PstI, AscI, AsiSI, BsaI, BsmBI, BbsI
discontinued: false
dominant: false
group u list: _613_
m user id: 0
owner id: 10379
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BsmBI site (cgtctcn/ and /nnnnngagacg) before RBS and after gene coding sequence that can be used to cut out RBS-gene for placement after other promoters. Sites for AsiSI (gcgat/cgc) and AscI (gg/cgcgcc) that can be used to insert existing oligos. Sites flanking these for BsaI (ggtctcn/ and /nnnnngagacc) that can be used to insert new oligos without the need for gel purification. A site for BbsI (gaagacnn/ and /nnnnnngtcttc) that produces a sticky end complementary to the first BsaI site, for use in generating a near wild type version of the gene.
star: false
creator: Cody Barta
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: pLacI
sequence annotation: BsmBI
sequence annotation: B0030
sequence annotation: T7 RNA Polymerase
sequence annotation: Start RNAP
sequence annotation: BsaI
sequence annotation: AsisI
sequence annotation: Asc1
sequence annotation: BbsI
sequence annotation: Stop RNAP
sequence: https://synbiohub.org/public/igem/BBa_J119009_sequence/1

pLacI
type: SequenceAnnotation
persistent identity: annotation2169622
display id: annotation2169622
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169622/range2169622/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J119009/annotation2169622/range2169622/1
type: Range
persistent identity: range2169622
display id: range2169622
version: 1
top level: BBa_J119009
direction: direction/forward
start: 1
end: 56
orientation: inline

BsmBI
type: SequenceAnnotation
persistent identity: annotation2169623
display id: annotation2169623
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169623/range2169623/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J119009/annotation2169623/range2169623/1
type: Range
persistent identity: range2169623
display id: range2169623
version: 1
top level: BBa_J119009
direction: direction/forward
start: 57
end: 62
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation2169624
display id: annotation2169624
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169624/range2169624/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J119009/annotation2169624/range2169624/1
type: Range
persistent identity: range2169624
display id: range2169624
version: 1
top level: BBa_J119009
direction: direction/forward
start: 68
end: 82
orientation: inline

T7 RNA Polymerase
type: SequenceAnnotation
persistent identity: annotation2169633
display id: annotation2169633
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169633/range2169633/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J119009/annotation2169633/range2169633/1
type: Range
persistent identity: range2169633
display id: range2169633
version: 1
top level: BBa_J119009
direction: direction/forward
start: 89
end: 2788
orientation: inline

Start RNAP
type: SequenceAnnotation
persistent identity: annotation2169625
display id: annotation2169625
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169625/range2169625/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J119009/annotation2169625/range2169625/1
type: Range
persistent identity: range2169625
display id: range2169625
version: 1
top level: BBa_J119009
direction: direction/forward
start: 89
end: 91
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2169626
display id: annotation2169626
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169626/range2169626/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J119009/annotation2169626/range2169626/1
type: Range
persistent identity: range2169626
display id: range2169626
version: 1
top level: BBa_J119009
direction: direction/reverse
start: 99
end: 104
orientation: inline

AsisI
type: SequenceAnnotation
persistent identity: annotation2169627
display id: annotation2169627
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169627/range2169627/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J119009/annotation2169627/range2169627/1
type: Range
persistent identity: range2169627
display id: range2169627
version: 1
top level: BBa_J119009
direction: direction/forward
start: 107
end: 114
orientation: inline

Asc1
type: SequenceAnnotation
persistent identity: annotation2169628
display id: annotation2169628
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169628/range2169628/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J119009/annotation2169628/range2169628/1
type: Range
persistent identity: range2169628
display id: range2169628
version: 1
top level: BBa_J119009
direction: direction/reverse
start: 117
end: 124
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2169629
display id: annotation2169629
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169629/range2169629/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J119009/annotation2169629/range2169629/1
type: Range
persistent identity: range2169629
display id: range2169629
version: 1
top level: BBa_J119009
direction: direction/reverse
start: 126
end: 131
orientation: inline

BbsI
type: SequenceAnnotation
persistent identity: annotation2169630
display id: annotation2169630
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169630/range2169630/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J119009/annotation2169630/range2169630/1
type: Range
persistent identity: range2169630
display id: range2169630
version: 1
top level: BBa_J119009
direction: direction/forward
start: 137
end: 142
orientation: inline

Stop RNAP
type: SequenceAnnotation
persistent identity: annotation2169632
display id: annotation2169632
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169632/range2169632/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J119009/annotation2169632/range2169632/1
type: Range
persistent identity: range2169632
display id: range2169632
version: 1
top level: BBa_J119009
direction: direction/forward
start: 2789
end: 2791
orientation: inline

BsmBI
type: SequenceAnnotation
persistent identity: annotation2169631
display id: annotation2169631
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169631/range2169631/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J119009/annotation2169631/range2169631/1
type: Range
persistent identity: range2169631
display id: range2169631
version: 1
top level: BBa_J119009
direction: direction/reverse
start: 2800
end: 2805
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

BBa_I732017
type: ComponentDefinition
persistent identity: BBa I732017
display id: BBa_I732017
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ full-length (RBS+ TERM-)
created: 2007-07-13T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: I732006 inserted into pSB1A2-B0034
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: None
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_I732005
sequence annotation: BBa_B0034
sequence annotation: BBa_I732005
sequence: https://synbiohub.org/public/igem/BBa_I732017_sequence/1

BBa_B0034
type: Component
persistent identity: component1938189
display id: component1938189
version: 1
top level: BBa_I732017
definition: BBa_B0034
access: public

BBa_I732005
type: Component
persistent identity: component1938194
display id: component1938194
version: 1
top level: BBa_I732017
definition: LacZ
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1938189
display id: annotation1938189
version: 1
top level: BBa_I732017
location: https://synbiohub.org/public/igem/BBa_I732017/annotation1938189/range1938189/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732017/annotation1938189/range1938189/1
type: Range
persistent identity: range1938189
display id: range1938189
version: 1
top level: BBa_I732017
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_I732005
type: SequenceAnnotation
persistent identity: annotation1938194
display id: annotation1938194
version: 1
top level: BBa_I732017
location: https://synbiohub.org/public/igem/BBa_I732017/annotation1938194/range1938194/1
component: BBa_I732005

https://synbiohub.org/public/igem/BBa_I732017/annotation1938194/range1938194/1
type: Range
persistent identity: range1938194
display id: range1938194
version: 1
top level: BBa_I732017
direction: direction/reverse
start: 19
end: 3093
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacZ
type: ComponentDefinition
persistent identity: BBa I732005
display id: BBa_I732005
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ (encoding beta-galactosidase, full-length)
created: 2007-07-05T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified and standardized from BL21
owned by: james
owned by: myers
mutable description: LacZ encodes beta-galactosidase, an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose. Beta-galactosidase can also cleaves X-gal and ONPG into colorful products.
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: C->T
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732005_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936871
display id: annotation1936871
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I732005/annotation1936871/range1936871/1
type: Range
persistent identity: range1936871
display id: range1936871
version: 1
top level: LacZ
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936870
display id: annotation1936870
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732005/annotation1936870/range1936870/1
type: Range
persistent identity: range1936870
display id: range1936870
version: 1
top level: LacZ
direction: direction/forward
start: 1
end: 3075
orientation: inline

C->T
type: SequenceAnnotation
persistent identity: annotation1936869
display id: annotation1936869
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_I732005/annotation1936869/range1936869/1
type: Range
persistent identity: range1936869
display id: range1936869
version: 1
top level: LacZ
direction: direction/forward
start: 3024
end: 3024
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936872
display id: annotation1936872
version: 1
top level: LacZ
location: https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732005/annotation1936872/range1936872/1
type: Range
persistent identity: range1936872
display id: range1936872
version: 1
top level: LacZ
direction: direction/forward
start: 3073
end: 3075
orientation: inline

https://synbiohub.org/public/igem/BBa_J119012_sequence/1
type: Sequence
persistent identity: BBa J119012 sequence
display id: BBa_J119012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J119000_sequence/1
type: Sequence
persistent identity: BBa J119000 sequence
display id: BBa_J119000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J119009_sequence/1
type: Sequence
persistent identity: BBa J119009 sequence
display id: BBa_J119009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgcgatcgcgaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732017_sequence/1
type: Sequence
persistent identity: BBa I732017 sequence
display id: BBa_I732017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732005_sequence/1
type: Sequence
persistent identity: BBa I732005 sequence
display id: BBa_I732005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtcgtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgccgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggacagtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgctggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataaaccgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaagttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcggcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcgaaaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctgattgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgttgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcaggatatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctgtgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccgatgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgatcatctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatccttcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtggatgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgctgatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtatccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggtcggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcacgccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtgaccagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaagcgg…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z