LOCUS BBa_J119016 3568 bp DNA linear 09-Dec-2025 DEFINITION pLac-RBS-T7RNAP-pT7-RBS-RFP ACCESSION BBa_J119016 VERSION BBa_J119016.1 FEATURES Location/Qualifiers promoter 1..56 /label=pLacI misc_feature 57..62 /label=BsmBI RBS 68..82 /label=B0030 misc_feature 89..91 /label=Start RNAP misc_feature 99..104 /label=BsaI misc_feature 107..114 /label=AsisI misc_feature 117..124 /label=Asc1 misc_feature 126..131 /label=BsaI misc_feature 137..142 /label=BbsI misc_feature 2800..2805 /label=BsmBI misc_feature 2789..2791 /label=Stop RNAP CDS 89..2788 /label=T7 RNA Polymerase CDS 1..2805 /label=BBa_J119009 misc_feature 2831..2831 /label=Transcription Start Site misc_feature 2814..2824 /label=Polymerase Binding Region misc_feature 2825..2836 /label=Initiation Region promoter 2814..2836 /label=BBa_R0085 misc_feature 2849..2852 /label=conserved RBS 2845..2856 /label=BBa_B0034 CDS 2863..3537 /label=mrfp1 misc_feature 3544..3568 /label=Help:Barcodes CDS 2863..3568 /label=BBa_E1010 misc_feature 2814..3568 /label=BBa_K199118 ORIGIN 1 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggcgtc 61 tcggcgaatt aaagaggaga aagtaggcat gaacacgaga gaccacgcga tcgcgaggcg 121 cgccaggtct ctgctggaag acgtcacgat taacatcgct aagaacgact tctctgacat 181 cgaactggct gctatcccgt tcaacactct agctgaccat tacggtgagc gtttagctcg 241 cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat 301 gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat 361 cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc 421 taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt 481 agcgtacatc accattaaga ccactctagc ttgcctaacc agtgctgaca atacaaccgt 541 tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat 601 ccgtgacctt gaagctaagc acttcaagaa aaacgtcgag gaacaactca acaagcgcgt 661 agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg 721 tctactcggt ggcgaggcgt ggtcctcgtg gcataaggag gactctattc atgtaggagt 781 acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc 841 tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat 901 cgcaacccgt gcaggggcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc 961 tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct 1021 agcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaggacg tttacatgcc 1081 tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt 1141 cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc 1201 gattgagcgt gaagaactcc cgatgaaacc ggaggacatc gacatgaatc ctgaggctct 1261 caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg 1321 ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat 1381 ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc 1441 gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa 1501 ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc 1561 gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa 1621 gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt 1681 ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct 1741 ggcgtttgac gggtcttgct ccggcatcca gcacttctcc gcgatgctcc gagatgaggt 1801 aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt 1861 tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt 1921 agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa 1981 ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt 2041 catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac 2101 cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc 2161 tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga 2221 agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa 2281 aactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc 2341 tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca 2401 gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca 2461 ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac 2521 tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt 2581 cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga 2641 cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca 2701 cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga 2761 catcttagag tcggacttcg cgttcgcgta ataacgcatg agacgtacta gagtaatacg 2821 actcactata gggagatact agagaaagag gagaaatact agatggcttc ctccgaagac 2881 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag 2941 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg 3001 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag 3061 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc 3121 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc 3181 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt 3241 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc 3301 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa 3361 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg 3421 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa 3481 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa 3541 taacgctgat agtgctagtg tagatcgc //