BBa_J119016
type: ComponentDefinition
persistent identity: BBa J119016
display id: BBa_J119016
version: 1
was generated by: iGEM to SBOL conversion
description: pLac-RBS-T7RNAP-pT7-RBS-RFP
created: 2011-10-06T11:00:00Z
modified: 2015-08-31T04:08:26Z
mutable provenance: The pLac-RBS-T7RNAP gene came from geneart DNA synthesis. The pT7-RBS-GFP came from iGEM09_MoWestern_Davidson.
owned by: james
owned by: myers
mutable description: This gene was created to be a self promoter. The T7RNAP gene product is T7RNAP which binds to the pT7 promoter and cause the cell to fluoresce red.
discontinued: false
dominant: false
group u list: _613_
m user id: 0
owner id: 10415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The gene produces a red color but we have speculated that this is caused by read through transcription.
star: false
creator: Michel Conn
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K199118
component: BBa_J119009
sequence annotation: BBa_J119009
sequence annotation: BBa_K199118
sequence: https://synbiohub.org/public/igem/BBa_J119016_sequence/1

BBa_K199118
type: Component
persistent identity: component2250810
display id: component2250810
version: 1
top level: BBa_J119016
definition: BBa_K199118
access: public

BBa_J119009
type: Component
persistent identity: component2250800
display id: component2250800
version: 1
top level: BBa_J119016
definition: BBa_J119009
access: public

BBa_J119009
type: SequenceAnnotation
persistent identity: annotation2250800
display id: annotation2250800
version: 1
top level: BBa_J119016
location: https://synbiohub.org/public/igem/BBa_J119016/annotation2250800/range2250800/1
component: BBa_J119009

https://synbiohub.org/public/igem/BBa_J119016/annotation2250800/range2250800/1
type: Range
persistent identity: range2250800
display id: range2250800
version: 1
top level: BBa_J119016
direction: direction/reverse
start: 1
end: 2805
orientation: inline

BBa_K199118
type: SequenceAnnotation
persistent identity: annotation2250810
display id: annotation2250810
version: 1
top level: BBa_J119016
location: https://synbiohub.org/public/igem/BBa_J119016/annotation2250810/range2250810/1
component: BBa_K199118

https://synbiohub.org/public/igem/BBa_J119016/annotation2250810/range2250810/1
type: Range
persistent identity: range2250810
display id: range2250810
version: 1
top level: BBa_J119016
direction: direction/reverse
start: 2814
end: 3568
orientation: inline

BBa_K199118
type: ComponentDefinition
persistent identity: BBa K199118
display id: BBa_K199118
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + RFP
created: 2010-03-02T12:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: Biobrick parts.
owned by: james
owned by: myers
mutable description: T7 Promoter (R0085) + RBS (B0034) + RFP (E1010)
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5118
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Clifton Davis
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_R0085
component: BBa_E1010
sequence annotation: BBa_R0085
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K199118_sequence/1

BBa_B0034
type: Component
persistent identity: component2247070
display id: component2247070
version: 1
top level: BBa_K199118
definition: BBa_B0034
access: public

BBa_R0085
type: Component
persistent identity: component2247068
display id: component2247068
version: 1
top level: BBa_K199118
definition: BBa_R0085
access: public

BBa_E1010
type: Component
persistent identity: component2247073
display id: component2247073
version: 1
top level: BBa_K199118
definition: mRFP1
access: public

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation2247068
display id: annotation2247068
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247068/range2247068/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_K199118/annotation2247068/range2247068/1
type: Range
persistent identity: range2247068
display id: range2247068
version: 1
top level: BBa_K199118
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2247070
display id: annotation2247070
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247070/range2247070/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K199118/annotation2247070/range2247070/1
type: Range
persistent identity: range2247070
display id: range2247070
version: 1
top level: BBa_K199118
direction: direction/reverse
start: 32
end: 43
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247073
display id: annotation2247073
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247073/range2247073/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K199118/annotation2247073/range2247073/1
type: Range
persistent identity: range2247073
display id: range2247073
version: 1
top level: BBa_K199118
direction: direction/reverse
start: 50
end: 755
orientation: inline

BBa_J119009
type: ComponentDefinition
persistent identity: BBa J119009
display id: BBa_J119009
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI-RBS-T7RNAP with sites for oligo insertion
created: 2011-07-26T11:00:00Z
modified: 2015-08-31T04:08:26Z
mutable provenance: GeneArt
owned by: james
owned by: myers
mutable description: J23100 promoter and B0030 RBS upstream of T7 RNAP gene. The following restriction sites do not occur in the promoter-RBS-T7 RNAP sequence. Some of them did not occur in the original sequence and others were removed while conserving the amino acid sequence. Both the forward and reverse complement were checked for type IIs enzyme sites. EcoRI, XbaI, SpeI, PstI, AscI, AsiSI, BsaI, BsmBI, BbsI
discontinued: false
dominant: false
group u list: _613_
m user id: 0
owner id: 10379
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BsmBI site (cgtctcn/ and /nnnnngagacg) before RBS and after gene coding sequence that can be used to cut out RBS-gene for placement after other promoters. Sites for AsiSI (gcgat/cgc) and AscI (gg/cgcgcc) that can be used to insert existing oligos. Sites flanking these for BsaI (ggtctcn/ and /nnnnngagacc) that can be used to insert new oligos without the need for gel purification. A site for BbsI (gaagacnn/ and /nnnnnngtcttc) that produces a sticky end complementary to the first BsaI site, for use in generating a near wild type version of the gene.
star: false
creator: Cody Barta
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: pLacI
sequence annotation: BsmBI
sequence annotation: B0030
sequence annotation: T7 RNA Polymerase
sequence annotation: Start RNAP
sequence annotation: BsaI
sequence annotation: AsisI
sequence annotation: Asc1
sequence annotation: BbsI
sequence annotation: Stop RNAP
sequence: https://synbiohub.org/public/igem/BBa_J119009_sequence/1

pLacI
type: SequenceAnnotation
persistent identity: annotation2169622
display id: annotation2169622
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169622/range2169622/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J119009/annotation2169622/range2169622/1
type: Range
persistent identity: range2169622
display id: range2169622
version: 1
top level: BBa_J119009
direction: direction/forward
start: 1
end: 56
orientation: inline

BsmBI
type: SequenceAnnotation
persistent identity: annotation2169623
display id: annotation2169623
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169623/range2169623/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J119009/annotation2169623/range2169623/1
type: Range
persistent identity: range2169623
display id: range2169623
version: 1
top level: BBa_J119009
direction: direction/forward
start: 57
end: 62
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation2169624
display id: annotation2169624
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169624/range2169624/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J119009/annotation2169624/range2169624/1
type: Range
persistent identity: range2169624
display id: range2169624
version: 1
top level: BBa_J119009
direction: direction/forward
start: 68
end: 82
orientation: inline

T7 RNA Polymerase
type: SequenceAnnotation
persistent identity: annotation2169633
display id: annotation2169633
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169633/range2169633/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J119009/annotation2169633/range2169633/1
type: Range
persistent identity: range2169633
display id: range2169633
version: 1
top level: BBa_J119009
direction: direction/forward
start: 89
end: 2788
orientation: inline

Start RNAP
type: SequenceAnnotation
persistent identity: annotation2169625
display id: annotation2169625
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169625/range2169625/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J119009/annotation2169625/range2169625/1
type: Range
persistent identity: range2169625
display id: range2169625
version: 1
top level: BBa_J119009
direction: direction/forward
start: 89
end: 91
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2169626
display id: annotation2169626
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169626/range2169626/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J119009/annotation2169626/range2169626/1
type: Range
persistent identity: range2169626
display id: range2169626
version: 1
top level: BBa_J119009
direction: direction/reverse
start: 99
end: 104
orientation: inline

AsisI
type: SequenceAnnotation
persistent identity: annotation2169627
display id: annotation2169627
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169627/range2169627/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J119009/annotation2169627/range2169627/1
type: Range
persistent identity: range2169627
display id: range2169627
version: 1
top level: BBa_J119009
direction: direction/forward
start: 107
end: 114
orientation: inline

Asc1
type: SequenceAnnotation
persistent identity: annotation2169628
display id: annotation2169628
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169628/range2169628/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J119009/annotation2169628/range2169628/1
type: Range
persistent identity: range2169628
display id: range2169628
version: 1
top level: BBa_J119009
direction: direction/reverse
start: 117
end: 124
orientation: inline

BsaI
type: SequenceAnnotation
persistent identity: annotation2169629
display id: annotation2169629
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169629/range2169629/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J119009/annotation2169629/range2169629/1
type: Range
persistent identity: range2169629
display id: range2169629
version: 1
top level: BBa_J119009
direction: direction/reverse
start: 126
end: 131
orientation: inline

BbsI
type: SequenceAnnotation
persistent identity: annotation2169630
display id: annotation2169630
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169630/range2169630/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J119009/annotation2169630/range2169630/1
type: Range
persistent identity: range2169630
display id: range2169630
version: 1
top level: BBa_J119009
direction: direction/forward
start: 137
end: 142
orientation: inline

Stop RNAP
type: SequenceAnnotation
persistent identity: annotation2169632
display id: annotation2169632
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169632/range2169632/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J119009/annotation2169632/range2169632/1
type: Range
persistent identity: range2169632
display id: range2169632
version: 1
top level: BBa_J119009
direction: direction/forward
start: 2789
end: 2791
orientation: inline

BsmBI
type: SequenceAnnotation
persistent identity: annotation2169631
display id: annotation2169631
version: 1
top level: BBa_J119009
location: https://synbiohub.org/public/igem/BBa_J119009/annotation2169631/range2169631/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J119009/annotation2169631/range2169631/1
type: Range
persistent identity: range2169631
display id: range2169631
version: 1
top level: BBa_J119009
direction: direction/reverse
start: 2800
end: 2805
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

https://synbiohub.org/public/igem/BBa_J119016_sequence/1
type: Sequence
persistent identity: BBa J119016 sequence
display id: BBa_J119016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgcgatcgcgaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199118_sequence/1
type: Sequence
persistent identity: BBa K199118 sequence
display id: BBa_K199118_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J119009_sequence/1
type: Sequence
persistent identity: BBa J119009 sequence
display id: BBa_J119009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgcgatcgcgaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z