LOCUS       BBa_J119017             3568 bp    DNA     linear       16-Feb-2025
DEFINITION  RFP-RBS-pT7-pLac-RBS-T7RNAP
ACCESSION   BBa_J119017
VERSION     BBa_J119017.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..724
                     /label=mrfp1
     misc_feature    731..755
                     /label=Help:Barcodes
     CDS             50..755
                     /label=BBa_E1010
     misc_feature    1..755
                     /label=BBa_K199118
     promoter        764..819
                     /label=pLacI
     misc_feature    820..825
                     /label=BsmBI
     RBS             831..845
                     /label=B0030
     misc_feature    852..854
                     /label=Start RNAP
     misc_feature    862..867
                     /label=BsaI
     misc_feature    870..877
                     /label=AsisI
     misc_feature    880..887
                     /label=Asc1
     misc_feature    889..894
                     /label=BsaI
     misc_feature    900..905
                     /label=BbsI
     misc_feature    3563..3568
                     /label=BsmBI
     misc_feature    3552..3554
                     /label=Stop RNAP
     CDS             852..3551
                     /label=T7 RNA Polymerase
     CDS             764..3568
                     /label=BBa_J119009
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      541 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      601 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      661 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      721 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagcgttgac accatcgaat
      781 ggcgcaaaac ctttcgcggt atggcatgat agcgcccggc gtctcggcga attaaagagg
      841 agaaagtagg catgaacacg agagaccacg cgatcgcgag gcgcgccagg tctctgctgg
      901 aagacgtcac gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc
      961 cgttcaacac tctagctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg
     1021 agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta
     1081 aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc ctactcccta
     1141 agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc
     1201 cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta
     1261 agaccactct agcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg
     1321 caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta
     1381 agcacttcaa gaaaaacgtc gaggaacaac tcaacaagcg cgtagggcac gtctacaaga
     1441 aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg
     1501 cgtggtcctc gtggcataag gaggactcta ttcatgtagg agtacgctgc atcgagatgc
     1561 tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag
     1621 actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcagggg
     1681 cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg
     1741 gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctagcgctg gtgcgtactc
     1801 acagtaagaa agcactgatg cgctacgagg acgtttacat gcctgaggtg tacaaagcga
     1861 ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg
     1921 taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac
     1981 tcccgatgaa accggaggac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg
     2041 ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt
     2101 tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca
     2161 tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga
     2221 ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc
     2281 tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct gagcgcatca
     2341 agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca
     2401 cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg
     2461 gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt
     2521 gctccggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta
     2581 acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg
     2641 agattctaca agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg
     2701 agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat
     2761 ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg
     2821 ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg
     2881 attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc
     2941 tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta
     3001 agtctgctgc taagctgctg gctgctgagg tcaaagataa gaaaactgga gagattcttc
     3061 gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca
     3121 agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc ttacagccta
     3181 ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc
     3241 ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg
     3301 agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc attccggctg
     3361 acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg
     3421 atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca
     3481 aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact
     3541 tcgcgttcgc gtaataacgc atgagacg
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