##gff-version 3
##sequence-region BBa_J119019 1 3568
BBa_J119019	.	CDS	764	3568	.	+	0	ID=BBa_J119009;Name=BBa_J119009
BBa_J119019	.	sequence_feature	870	877	.	+	0	ID=annotation2169627;Name=AsisI;Parent=BBa_J119009
BBa_J119019	.	sequence_feature	3563	3568	.	+	0	ID=annotation2169631;Name=BsmBI;Parent=BBa_J119009
BBa_J119019	.	sequence_feature	862	867	.	+	0	ID=annotation2169626;Name=BsaI;Parent=BBa_J119009
BBa_J119019	.	stop_codon	3552	3554	.	+	0	ID=annotation2169632;Name=Stop RNAP;Parent=BBa_J119009
BBa_J119019	.	sequence_feature	900	905	.	+	0	ID=annotation2169630;Name=BbsI;Parent=BBa_J119009
BBa_J119019	.	start_codon	852	854	.	+	0	ID=annotation2169625;Name=Start RNAP;Parent=BBa_J119009
BBa_J119019	.	ribosome_entry_site	831	845	.	+	0	ID=annotation2169624;Name=B0030;Parent=BBa_J119009
BBa_J119019	.	promoter	764	819	.	+	0	ID=annotation2169622;Name=pLacI;Parent=BBa_J119009
BBa_J119019	.	sequence_feature	889	894	.	+	0	ID=annotation2169629;Name=BsaI;Parent=BBa_J119009
BBa_J119019	.	sequence_feature	820	825	.	+	0	ID=annotation2169623;Name=BsmBI;Parent=BBa_J119009
BBa_J119019	.	CDS	852	3551	.	+	0	ID=annotation2169633;Name=T7 RNA Polymerase;Parent=BBa_J119009
BBa_J119019	.	sequence_feature	880	887	.	+	0	ID=annotation2169628;Name=Asc1;Parent=BBa_J119009
BBa_J119019	.	engineered_region	1	755	.	+	0	ID=BBa_J119018;Name=BBa_J119018
BBa_J119019	.	stem_loop	712	724	.	+	0	ID=annotation2158196;Name=RBS34rev;Parent=BBa_J119018
BBa_J119019	.	promoter	732	755	.	+	0	ID=annotation2158197;Name=pTetrev;Parent=BBa_J119018
BBa_J119019	.	sequence_feature	21	705	.	+	0	ID=annotation2158195;Name=mRFP1rev;Parent=BBa_J119018
BBa_J119019	.	sequence_feature	1	15	.	+	0	ID=annotation2158194;Name=Barcode;Parent=BBa_J119018
>BBa_J119019
gcgatctacactagcactatcagcgttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgat
ggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggtttttta
gccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttca
gagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcgga
cgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaaca
acaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgt
cagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggacc
acctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcg
tgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagt
atttctcctctttctctagtatctccctatagtgagtcgtattatactagagcgttgacaccatcgaatggcgcaaaac
ctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccac
gcgatcgcgaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaac
tggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatga
gtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgct
gccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagcta
agcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagac
cactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggac
gaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcg
tagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggc
gtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatg
gttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgagg
ctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggac
tggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactg
atgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaaca
agaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtga
agaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtg
taccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaacc
ataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaa
cgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccac
ggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatca
tggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctt
tgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctccggc
atccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcagg
acatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagt
taccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctg
gcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtc
aacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgc
tggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaag
tctgctgctaagctgctggctgctgaggtcaaagataagaaaactggagagattcttcgcaagcgttgcgctgtgcatt
gggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcgg
tcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgct
cctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaat
cttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactat
ggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattg
gacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaat
aacgcatgagacg