LOCUS       BBa_J119023              944 bp    DNA     linear       22-Dec-2024
DEFINITION  RFP GGA BD18 destination vector - BbsI and BsaI
ACCESSION   BBa_J119023
VERSION     BBa_J119023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=BBa_prefix
     misc_feature    34..39
                     /label=BsaI cuts left
     stem_loop       40..134
                     /label=TT B0014
     misc_feature    135..140
                     /label=BsaI cuts right
     misc_feature    29..32
                     /label=BsaI Word 1
     misc_feature    142..145
                     /label=BsaI Word 2
     CDS             158..245
                     /label=BD18 bicistron
     misc_feature    241..243
                     /label=stop for BD18
     misc_feature    243..245
                     /label=Start GOI
     CDS             243..923
                     /label=RFP E1010 with 4 pt mutations
     misc_feature    918..923
                     /label=Stop E1010
     misc_feature    924..944
                     /label=BBa_suffix
     misc_feature    23..28
                     /label=BbsI cuts left
     misc_feature    17..20
                     /label=BbsI Word 1
     misc_feature    146..151
                     /label=BbsI cuts right
     misc_feature    154..157
                     /label=BbsI Word 2
     misc_feature    260..260
                     /label=C--> T for E. coli
     misc_feature    545..545
                     /label=C--> T for E. coli
     misc_feature    704..704
                     /label=C--> T for E. coli
     misc_feature    863..863
                     /label=C--> T for E. coli
     RBS             175..183
                     /label=leader RBS
     RBS             228..236
                     /label=GOI RBS
ORIGIN
        1 gaattcgcgg ccgcttctag aggtcttccg actgagacct cacactggct caccttcggg
       61 tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg
      121 cttttttatt atttggtctc agcgggaaga caactagggg cccaagttca cttaaaaagg
      181 agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgcgac ggagcgtttc
      241 taatggcttc ctccgaagat gttatcaaag agttcatgcg tttcaaagtt cgtatggaag
      301 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag
      361 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca
      421 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc
      481 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg
      541 aagatggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct
      601 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa
      661 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agatggtgct ctgaaaggtg
      721 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
      781 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg
      841 acatcacctc ccacaacgaa gattacacca tcgttgaaca gtacgaacgt gctgaaggtc
      901 gtcactccac cggtgcttaa taatactagt agcggccgct gcag
//