##gff-version 3
##sequence-region BBa_J119023 1 944
BBa_J119023	.	sequence_feature	154	157	.	+	0	ID=annotation2167732;Name=BbsI Word 2
BBa_J119023	.	sequence_feature	23	28	.	+	0	ID=annotation2167729;Name=BbsI cuts left
BBa_J119023	.	point_mutation	863	863	.	+	0	ID=annotation2167736;Name=C--> T for E. coli
BBa_J119023	.	CDS	243	923	.	+	0	ID=annotation2167726;Name=RFP E1010 with 4 pt mutations
BBa_J119023	.	sequence_feature	146	151	.	+	0	ID=annotation2167731;Name=BbsI cuts right
BBa_J119023	.	point_mutation	704	704	.	+	0	ID=annotation2167735;Name=C--> T for E. coli
BBa_J119023	.	ribosome_entry_site	175	183	.	+	0	ID=annotation2167754;Name=leader RBS
BBa_J119023	.	sequence_feature	34	39	.	+	0	ID=annotation2167718;Name=BsaI cuts left
BBa_J119023	.	engineered_region	1	22	.	+	0	ID=annotation2167717;Name=BBa_prefix
BBa_J119023	.	CDS	158	245	.	+	0	ID=annotation2167723;Name=BD18 bicistron
BBa_J119023	.	start_codon	243	245	.	+	0	ID=annotation2167725;Name=Start GOI
BBa_J119023	.	sequence_feature	135	140	.	+	0	ID=annotation2167720;Name=BsaI cuts right
BBa_J119023	.	sequence_feature	142	145	.	+	0	ID=annotation2167722;Name=BsaI Word 2
BBa_J119023	.	sequence_feature	29	32	.	+	0	ID=annotation2167721;Name=BsaI Word 1
BBa_J119023	.	stop_codon	241	243	.	+	0	ID=annotation2167724;Name=stop for BD18
BBa_J119023	.	stem_loop	40	134	.	+	0	ID=annotation2167719;Name=TT B0014
BBa_J119023	.	point_mutation	545	545	.	+	0	ID=annotation2167734;Name=C--> T for E. coli
BBa_J119023	.	stop_codon	918	923	.	+	0	ID=annotation2167727;Name=Stop E1010
BBa_J119023	.	point_mutation	260	260	.	+	0	ID=annotation2167733;Name=C--> T for E. coli
BBa_J119023	.	engineered_region	924	944	.	+	0	ID=annotation2167728;Name=BBa_suffix
BBa_J119023	.	ribosome_entry_site	228	236	.	+	0	ID=annotation2167755;Name=GOI RBS
BBa_J119023	.	sequence_feature	17	20	.	+	0	ID=annotation2167730;Name=BbsI Word 1
>BBa_J119023
gaattcgcggccgcttctagaggtcttccgactgagacctcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagagagaatataaaaagccagattattaatccggcttttttattatttggtctcagcgggaagacaactagg
ggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgt
ttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtca
cgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggt
ggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctg
acatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgt
tgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccg
tccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatggtgctc
tgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggc
taaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagattacacc
atcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagtagcggccgctgcag