LOCUS       BBa_J119035              848 bp    DNA     linear       22-Dec-2024
DEFINITION  pTet+BD18+RFP
ACCESSION   BBa_J119035
VERSION     BBa_J119035.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             73..81
                     /label=leader RBS
     misc_feature    87..89
                     /label=Start
     misc_feature    138..140
                     /label=Stop
     misc_feature    140..142
                     /label=Start
     RBS             125..133
                     /label=GOI RBS
     misc_feature    55..142
                     /label=BBa_J119024
     CDS             143..817
                     /label=mrfp1
     misc_feature    824..848
                     /label=Help:Barcodes
     CDS             143..848
                     /label=BBa_E1010
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgggccc
       61 aagttcactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa catcttaatc
      121 atgcgacgga gcgtttctaa tgatggcttc ctccgaagac gttatcaaag agttcatgcg
      181 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      241 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      301 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      361 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      421 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      481 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      541 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      601 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      661 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      721 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      781 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
      841 tagatcgc
//