##gff-version 3
##sequence-region BBa_J119035 1 848
BBa_J119035	.	CDS	143	848	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_J119035	.	CDS	143	817	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J119035	.	engineered_tag	824	848	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J119035	.	engineered_region	55	142	.	+	0	ID=BBa_J119024;Name=BBa_J119024
BBa_J119035	.	start_codon	140	142	.	+	0	ID=annotation2167443;Name=Start;Parent=BBa_J119024
BBa_J119035	.	start_codon	87	89	.	+	0	ID=annotation2167441;Name=Start;Parent=BBa_J119024
BBa_J119035	.	stop_codon	138	140	.	+	0	ID=annotation2167442;Name=Stop;Parent=BBa_J119024
BBa_J119035	.	ribosome_entry_site	73	81	.	+	0	ID=annotation2167440;Name=leader RBS;Parent=BBa_J119024
BBa_J119035	.	ribosome_entry_site	125	133	.	+	0	ID=annotation2167444;Name=GOI RBS;Parent=BBa_J119024
BBa_J119035	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J119035	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J119035	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J119035	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J119035	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J119035	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_J119035
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgggcccaagttcacttaaaaaggag
atcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatgatggcttcctccgaag
acgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtga
aggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgg
gacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaac
tgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactc
ctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcag
aaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgc
gtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgcc
gggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgt
gctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc