LOCUS       BBa_J119036              850 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-BD18-RFP
ACCESSION   BBa_J119036
VERSION     BBa_J119036.1  
FEATURES             Location/Qualifiers
     promoter        17..22
                     /label=-35
     promoter        44..49
                     /label=-10
     promoter        1..56
                     /label=BBa_K091110
     RBS             75..83
                     /label=leader RBS
     misc_feature    89..91
                     /label=Start
     misc_feature    140..142
                     /label=Stop
     misc_feature    142..144
                     /label=Start
     RBS             127..135
                     /label=GOI RBS
     misc_feature    57..144
                     /label=BBa_J119024
     CDS             145..819
                     /label=mrfp1
     misc_feature    826..850
                     /label=Help:Barcodes
     CDS             145..850
                     /label=BBa_E1010
ORIGIN
        1 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccgggggc
       61 ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga aacatcttaa
      121 tcatgcgacg gagcgtttct aatgatggct tcctccgaag acgttatcaa agagttcatg
      181 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      241 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      301 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      361 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
      421 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
      481 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
      541 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
      601 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
      661 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
      721 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
      781 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag
      841 tgtagatcgc
//