LOCUS       BBa_J119043             2240 bp    DNA     linear       23-Dec-2024
DEFINITION  pSB1A8
ACCESSION   BBa_J119043
VERSION     BBa_J119043.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=suffix
     misc_feature    2207..2228
                     /label=prefix
     misc_feature    22..205
                     /label=B0025 terminator
     misc_feature    2167..2206
                     /label=B1010 terminator
     primer_bind     208..227
                     /label=VR primer
     primer_bind     2069..2088
                     /label=VF2 primer
     misc_feature    310..923
                     /label=pMB1 replication origin (rev)
     CDS             1085..1946
                     /label=Amp Resistance (rev)
     misc_feature    2228..2240
                     /label=stuffer
     misc_feature    1227..1227
                     /label=mutation A --> G removes BsaI site
ORIGIN
        1 tactagtagc ggccgctgca gtataaacgc agaaaggccc acccgaaggt gagccagtgt
       61 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
      121 actgagcctt tcgttttatt tgatgcctgg gcttcctcgc tcactgactc gctgcgctcg
      181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
      781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1081 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1141 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1201 ggccccagtg ctgcaatgat accgcgggac ccacgctcac cggctccaga tttatcagca
     1261 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1321 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1381 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1441 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1501 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1561 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1621 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1681 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1741 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1801 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1861 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1921 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1981 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2041 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2101 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatga
     2161 tttctgcgcc gcaaaccccg cccctgacag ggcggggttt cgccgcgaat tcgcggccgc
     2221 ttctagaggc atacagtcgt
//