##gff-version 3
##sequence-region BBa_J119044 1 926
BBa_J119044	.	ribosome_entry_site	210	218	.	+	0	ID=annotation2180013;Name=RFP RBS
BBa_J119044	.	sequence_feature	28	33	.	+	0	ID=annotation2180007;Name=BsaI cuts left
BBa_J119044	.	sequence_feature	136	139	.	+	0	ID=annotation2180010;Name=BsaI sticky end right
BBa_J119044	.	start_codon	225	227	.	+	0	ID=annotation2180015;Name=Start RFP
BBa_J119044	.	engineered_region	1	22	.	+	0	ID=annotation2180005;Name=Bba prefix
BBa_J119044	.	ribosome_entry_site	157	165	.	+	0	ID=annotation2180012;Name=leader RBS
BBa_J119044	.	stop_codon	223	225	.	+	0	ID=annotation2180014;Name=Stop for BD18
BBa_J119044	.	stop_codon	900	905	.	+	0	ID=annotation2180017;Name=Double Stop
BBa_J119044	.	sequence_feature	23	26	.	+	0	ID=annotation2180006;Name=BsaI sticky end left
BBa_J119044	.	sequence_feature	140	227	.	+	0	ID=annotation2180011;Name=BD18 bicistron
BBa_J119044	.	engineered_region	906	926	.	+	0	ID=annotation2180018;Name=BBa suffix
BBa_J119044	.	CDS	225	905	.	+	0	ID=annotation2180016;Name=RFP optimized for E. coli 
BBa_J119044	.	sequence_feature	129	134	.	+	0	ID=annotation2180009;Name=Bsa cuts right
BBa_J119044	.	stem_loop	34	128	.	+	0	ID=annotation2180008;Name=TT B0014
>BBa_J119044
gaattcgcggccgcttctagagcgactgagacctcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagagagaatataaaaagccagattattaatccggcttttttattatttggtctcagcgggggcccaagttcacttaaa
aaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatggcaagcagcg
aagatgtgatcaaagaatttatgcgtttcaaggtgcgtatggaaggtagcgttaatggtcatgaatttgaaattgaagg
tgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgca
tgggatattctgagtccgcagttccagtatggtagcaaagcatatgttaaacatccggcagatattccggattacctga
aactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccagga
tagcagcttacaggatggtgagtttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatg
cagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaa
tgcgtctgaagctgaaagacggtggtcattatgatgcagaagttaaaaccacctacatggccaaaaaaccggttcagct
gcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgtggaacagtatgaa
cgtgcagaaggtcgccatagtaccggtgcctaataatactagtagcggccgctgcag