LOCUS       BBa_J119045             2167 bp    DNA     linear       23-Dec-2024
DEFINITION  pSB1C8
ACCESSION   BBa_J119045
VERSION     BBa_J119045.1  
FEATURES             Location/Qualifiers
     misc_feature    2134..2155
                     /label=Prefix
     misc_feature    1..21
                     /label=Suffix
     primer_bind     208..227
                     /label=VR primer
     primer_bind     1984..2003
                     /label=VF2
     CDS             1213..1872
                     /label=Chloramphenicol resistance
     misc_feature    313..927
                     /label=pMB1 replication origin
     misc_feature    22..205
                     /label=B0025 terminator
     misc_feature    2094..2133
                     /label=B0010 terminator
     misc_feature    2155..2167
                     /label=stuffer
ORIGIN
        1 tactagtagc ggccgctgca gtataaacgc agaaaggccc acccgaaggt gagccagtgt
       61 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
      121 actgagcctt tcgttttatt tgatgcctgg gcttcctcgc tcactgactc gctgcgctcg
      181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      301 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac
      361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1081 gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     1141 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     1201 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1261 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1321 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1381 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     1441 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1501 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1561 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1621 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     1681 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1741 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1801 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1861 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     1921 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc
     1981 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     2041 gtatcacgag gcagaatttc agataaaaaa aatgatttct gcgccgcaaa ccccgcccct
     2101 gacagggcgg ggtttcgccg cgaattcgcg gccgcttcta gaggcataca gtcgtgcatg
     2161 catgcat
//