LOCUS BBa_J119046 2289 bp DNA linear 22-Nov-2024 DEFINITION pSB1K8 ACCESSION BBa_J119046 VERSION BBa_J119046.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2256..2277 /label=Prefix primer_bind 208..227 /label=VR primer primer_bind 2118..2137 /label=VF2 primer stem_loop 22..205 /label=B0025 terminator stem_loop 2138..2215 /label=B0010 terminator misc_feature 2278..2289 /label=stuffer misc_feature 310..923 /label=pMB1 replication origin CDS 1148..1963 /label=Kanamycin resistance gene ORIGIN 1 tactagtagc ggccgctgca gtataaacgc agaaaggccc acccgaaggt gagccagtgt 61 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg 121 actgagcctt tcgttttatt tgatgcctgg gcttcctcgc tcactgactc gctgcgctcg 181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 301 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac 361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1081 gacagctcga gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca 1141 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat 1201 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc 1261 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa 1321 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag 1381 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa 1441 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc 1501 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag 1561 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat 1621 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc 1681 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca 1741 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt 1801 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt 1861 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta 1921 atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac 1981 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt 2041 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt 2101 tgaaggatca gctcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 2161 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatgat ttctgcgccg 2221 caaaccccgc ccctgacagg gcggggtttc gccgcgaatt cgcggccgct tctagaggca 2281 tacagtcgt //