LOCUS       BBa_J119046             2289 bp    DNA     linear       16-Jun-2024
DEFINITION  pSB1K8
ACCESSION   BBa_J119046
VERSION     BBa_J119046.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=Suffix
     misc_feature    2256..2277
                     /label=Prefix
     primer_bind     208..227
                     /label=VR primer
     primer_bind     2118..2137
                     /label=VF2 primer
     stem_loop       22..205
                     /label=B0025 terminator
     stem_loop       2138..2215
                     /label=B0010 terminator
     misc_feature    2278..2289
                     /label=stuffer
     misc_feature    310..923
                     /label=pMB1 replication origin
     CDS             1148..1963
                     /label=Kanamycin resistance gene
ORIGIN
        1 tactagtagc ggccgctgca gtataaacgc agaaaggccc acccgaaggt gagccagtgt
       61 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
      121 actgagcctt tcgttttatt tgatgcctgg gcttcctcgc tcactgactc gctgcgctcg
      181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      301 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac
      361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1081 gacagctcga gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca
     1141 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     1201 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     1261 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     1321 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     1381 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
     1441 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     1501 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     1561 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     1621 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     1681 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     1741 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     1801 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     1861 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     1921 atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     1981 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     2041 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     2101 tgaaggatca gctcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     2161 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatgat ttctgcgccg
     2221 caaaccccgc ccctgacagg gcggggtttc gccgcgaatt cgcggccgct tctagaggca
     2281 tacagtcgt
//