LOCUS       BBa_J119047             2546 bp    DNA     linear       28-Sep-2024
DEFINITION  pSB1T8
ACCESSION   BBa_J119047
VERSION     BBa_J119047.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=Suffix
     misc_feature    2513..2534
                     /label=Prefix
     primer_bind     208..227
                     /label=VR primer
     primer_bind     2375..2394
                     /label=VF2 primer
     stem_loop       22..205
                     /label=B0025 terminator
     stem_loop       2473..2512
                     /label=B0010 terminator
     misc_feature    314..928
                     /label=pMB1 replication origin
     CDS             1178..2368
                     /label=Tetracycline resistance gene
     misc_feature    2535..2546
                     /label=stuffer
     misc_feature    1831..1831
                     /label=c -> g BbsI site removed
ORIGIN
        1 tactagtagc ggccgctgca gtataaacgc agaaaggccc acccgaaggt gagccagtgt
       61 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
      121 actgagcctt tcgttttatt tgatgcctgg gcttcctcgc tcactgactc gctgcgctcg
      181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
      781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1081 gacagctcga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt
     1141 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
     1201 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact
     1261 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct
     1321 gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga
     1381 ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc
     1441 gatcatggcg accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat
     1501 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     1561 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     1621 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     1681 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     1741 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     1801 catgactatc gtcgccgcac ttatgactgt gttctttatc atgcaactcg taggacaggt
     1861 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     1921 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     1981 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     2041 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     2101 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     2161 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
     2221 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     2281 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
     2341 tgcatggagc cgggccacct cgacctaact cgagtgccac ctgacgtcta agaaaccatt
     2401 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     2461 aaatgatttc tgcgccgcaa accccgcccc tgacagggcg gggtttcgcc gcgaattcgc
     2521 ggccgcttct agaggcatac agtcgt
//