##gff-version 3
##sequence-region BBa_J119047 1 2546
BBa_J119047	.	engineered_region	1	21	.	+	0	ID=annotation2169696;Name=Suffix
BBa_J119047	.	stem_loop	22	205	.	+	0	ID=annotation2169700;Name=B0025 terminator
BBa_J119047	.	sequence_feature	2535	2546	.	+	0	ID=annotation2169741;Name=stuffer
BBa_J119047	.	primer_binding_site	2375	2394	.	+	0	ID=annotation2169699;Name=VF2 primer
BBa_J119047	.	CDS	1178	2368	.	+	0	ID=annotation2169703;Name=Tetracycline resistance gene
BBa_J119047	.	primer_binding_site	208	227	.	+	0	ID=annotation2169698;Name=VR primer
BBa_J119047	.	point_mutation	1831	1831	.	+	0	ID=annotation2169742;Name=c -> g BbsI site removed
BBa_J119047	.	sequence_feature	314	928	.	+	0	ID=annotation2169702;Name=pMB1 replication origin
BBa_J119047	.	engineered_region	2513	2534	.	+	0	ID=annotation2169697;Name=Prefix
BBa_J119047	.	stem_loop	2473	2512	.	+	0	ID=annotation2169701;Name=B0010 terminator
>BBa_J119047
tactagtagcggccgctgcagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgtt
caccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgggcttcctc
gctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatcc
acagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgt
tgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaaccc
gacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttacc
ggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt
aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcg
tcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtat
gtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc
tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttt
ttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgac
gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaa
attaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagattctcatgtttgac
agcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatc
taacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccg
ggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgc
aatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttgg
agccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatc
accggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggc
tcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgc
accattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataaggga
gagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccg
cacttatgactgtgttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccg
ctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtc
actggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtct
tgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgc
gttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagc
ctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatgga
ttgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaact
cgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatt
tcagataaaaaaaatgatttctgcgccgcaaaccccgcccctgacagggcggggtttcgccgcgaattcgcggccgctt
ctagaggcatacagtcgt