LOCUS BBa_J119048 3662 bp DNA linear 23-Dec-2024 DEFINITION pSB4A8 ACCESSION BBa_J119048 VERSION BBa_J119048.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 3629..3650 /label=Prefix primer_bind 208..227 /label=VR primer primer_bind 3491..3510 /label=VF2 primer CDS 2508..3368 /label=Ampicillin resistance gene misc_feature 314..2350 /label=I50042 low copy pSC101 origin stem_loop 22..205 /label=B0025 terminator stem_loop 3589..3628 /label=B0010 misc_feature 3651..3662 /label=stuffer ORIGIN 1 tactagtagc ggccgctgca gtataaacgc agaaaggccc acccgaaggt gagccagtgt 61 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg 121 actgagcctt tcgttttatt tgatgcctgg gcttcctcgc tcactgactc gctgcgctcg 181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 301 cgtaaaaagg ccgctgtcag accaagttta cgagctcgct tggactcctg ttgatagatc 361 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt 421 tttttattgg tgagaatcca agcactaggg acagtaagac gggtaagcct gttgatgata 481 ccgctgcctt actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt 541 ggtcagactg gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca 601 catagcagac ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg 661 ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact 721 gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg 781 atggtcggag acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa 841 gcctttaggg ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt 901 gtaatactgc ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt 961 tatctttttt tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa 1021 aacacacaaa ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc 1081 aaaggtctag cagaatttac agatacccac aactcaaagg aaaaggacat gtaattatca 1141 ttgactagcc catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat 1201 gtctgaatta gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga 1261 gcatgaaacc aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc 1321 tacaaggaaa gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat 1381 cagtagggaa aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac 1441 tgtggaaatc aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc 1501 caagttctca agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt 1561 ccagttaaaa aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata 1621 ctctatgagg atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc 1681 aaatatagag attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca 1741 tgagtttaaa aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta 1801 cagcaatatg aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca 1861 agttgaacta gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat 1921 gaatggtgac aaaataccaa caaccattac atcagattcc tacctacata acggactaag 1981 aaaaacacta cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt 2041 tttgagtgac atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa 2101 acaacgaacc acactagaga acatactggc taaatacgga aggatctgag gttcttatgg 2161 ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc 2221 gttacatatc aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag 2281 atacatcaga gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg 2341 acaatgtaac tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2401 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2461 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 2521 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 2581 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 2641 ataccgcggg acccacgctc accggctcca gatttatcag caataaacca gccagccgga 2701 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 2761 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 2821 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 2881 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 2941 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 3001 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 3061 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 3121 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 3181 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 3241 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 3301 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 3361 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 3421 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 3481 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 3541 aataggcgta tcacgaggca gaatttcaga taaaaaaaat gatttctgcg ccgcaaaccc 3601 cgcccctgac agggcggggt ttcgccgcga attcgcggcc gcttctagag gcatacagtc 3661 gt //