##gff-version 3
##sequence-region BBa_J119049 1 3577
BBa_J119049	.	point_mutation	208	227	.	+	0	ID=annotation2169718;Name=VR primer
BBa_J119049	.	sequence_feature	3566	3577	.	+	0	ID=annotation2169716;Name=stuffer
BBa_J119049	.	engineered_region	3544	3565	.	+	0	ID=annotation2169714;Name=Prefix
BBa_J119049	.	CDS	2633	3294	.	+	0	ID=annotation2169722;Name=Chloramphenicol resistance gene
BBa_J119049	.	stem_loop	3504	3543	.	+	0	ID=annotation2169720;Name=B0010 terminator
BBa_J119049	.	stem_loop	22	205	.	+	0	ID=annotation2169719;Name=B0025 terminator
BBa_J119049	.	sequence_feature	314	2350	.	+	0	ID=annotation2169721;Name=I50042 low copy pSC101 origin
BBa_J119049	.	engineered_region	1	21	.	+	0	ID=annotation2169715;Name=Suffix
BBa_J119049	.	primer_binding_site	3406	3425	.	+	0	ID=annotation2169717;Name=VF2 primer
>BBa_J119049
tactagtagcggccgctgcagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgtt
caccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgggcttcctc
gctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatcc
acagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgctg
tcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttc
agaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttg
atgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaa
aatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaa
gcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttaccc
ccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggag
acaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgta
gaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctggg
atctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacac
aaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatac
ccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctaga
ccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcat
gaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtat
cgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccag
agagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgcc
aagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataa
aatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaac
acaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactac
catgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattgg
tggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccga
acttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacgga
ctaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgc
aaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaa
atacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaac
tgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagag
cttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaactcttttctacggggtctgac
gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaa
attaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgaggcttggattctcacc
aataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaac
aggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcatt
ctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtat
aatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcac
ccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgcc
acatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagttt
gctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattc
cggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtcttt
aaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctt
tacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaa
tctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgcccgatc
aactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcag
aatttcagataaaaaaaatgatttctgcgccgcaaaccccgcccctgacagggcggggtttcgccgcgaattcgcggcc
gcttctagaggcatacagtcgt