LOCUS BBa_J119050 3712 bp DNA linear 16-Jun-2024 DEFINITION pSB4K8 ACCESSION BBa_J119050 VERSION BBa_J119050.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 3679..3700 /label=Prefix misc_feature 3701..3712 /label=stuffer primer_bind 3541..3560 /label=VF2 primer primer_bind 208..227 /label=VR primer stem_loop 22..205 /label=B0025 terminator stem_loop 3561..3638 /label=B0010 terminator misc_feature 310..2346 /label=I50042 low copy pSC101 origin CDS 2571..3386 /label=Kanamycin resistance gene ORIGIN 1 tactagtagc ggccgctgca gtataaacgc agaaaggccc acccgaaggt gagccagtgt 61 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg 121 actgagcctt tcgttttatt tgatgcctgg gcttcctcgc tcactgactc gctgcgctcg 181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 301 cgtaaaaagc tgtcagacca agtttacgag ctcgcttgga ctcctgttga tagatccagt 361 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt 421 tattggtgag aatccaagca ctagggacag taagacgggt aagcctgttg atgataccgc 481 tgccttactg ggtgcattag ccagtctgaa tgacctgtca cgggataatc cgaagtggtc 541 agactggaaa atcagagggc aggaactgct gaacagcaaa aagtcagata gcaccacata 601 gcagacccgc cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta 661 gtttccttgc atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca 721 gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg 781 tcggagacaa aaggaatatt cagcgatttg cccgagcttg cgagggtgct acttaagcct 841 ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa 901 tactgcggaa ctgactaaag tagtgagtta tacacagggc tgggatctat tctttttatc 961 tttttttatt ctttctttat tctataaatt ataaccactt gaatataaac aaaaaaaaca 1021 cacaaaggtc tagcggaatt tacagagggt ctagcagaat ttacaagttt tccagcaaag 1081 gtctagcaga atttacagat acccacaact caaaggaaaa ggacatgtaa ttatcattga 1141 ctagcccatc tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct 1201 gaattagttg ttttcaaagc aaatgaacta gcgattagtc gctatgactt aacggagcat 1261 gaaaccaagc taattttatg ctgtgtggca ctactcaacc ccacgattga aaaccctaca 1321 aggaaagaac ggacggtatc gttcacttat aaccaatacg ctcagatgat gaacatcagt 1381 agggaaaatg cttatggtgt attagctaaa gcaaccagag agctgatgac gagaactgtg 1441 gaaatcagga atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag 1501 ttctcaagcg aaaaattaga attagttttt agtgaagaga tattgcctta tcttttccag 1561 ttaaaaaaat tcataaaata taatctggaa catgttaagt cttttgaaaa caaatactct 1621 atgaggattt atgagtggtt attaaaagaa ctaacacaaa agaaaactca caaggcaaat 1681 atagagatta gccttgatga atttaagttc atgttaatgc ttgaaaataa ctaccatgag 1741 tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc 1801 aatatgaaat tggtggttga taagcgaggc cgcccgactg atacgttgat tttccaagtt 1861 gaactagata gacaaatgga tctcgtaacc gaacttgaga acaaccagat aaaaatgaat 1921 ggtgacaaaa taccaacaac cattacatca gattcctacc tacataacgg actaagaaaa 1981 acactacacg atgctttaac tgcaaaaatt cagctcacca gttttgaggc aaaatttttg 2041 agtgacatgc aaagtaagta tgatctcaat ggttcgttct catggctcac gcaaaaacaa 2101 cgaaccacac tagagaacat actggctaaa tacggaagga tctgaggttc ttatggctct 2161 tgtatctatc agtgaagcat caagactaac aaacaaaagt agaacaactg ttcaccgtta 2221 catatcaaag ggaaaactgt ccatatgcac agatgaaaac ggtgtaaaaa agatagatac 2281 atcagagctt ttacgagttt ttggtgcatt caaagctgtt caccatgaac agatcgacaa 2341 tgtaactttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 2401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 2461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgagtcccgt 2521 caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac 2581 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt 2641 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca 2701 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc 2761 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt 2821 gagaatggca aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc 2881 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg 2941 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg 3001 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat 3061 acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta 3121 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc 3181 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc 3241 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga 3301 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctggagcaa 3361 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac 3421 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga 3481 gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga tcagctcgag 3541 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 3601 tcacgaggca gaatttcaga taaaaaaaat gatttctgcg ccgcaaaccc cgcccctgac 3661 agggcggggt ttcgccgcga attcgcggcc gcttctagag gcatacagtc gt //