LOCUS BBa_J119051 3968 bp DNA linear 23-Dec-2024 DEFINITION pSB4T8 ACCESSION BBa_J119051 VERSION BBa_J119051.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 3935..3956 /label=Prefix misc_feature 3957..3968 /label=stuffer primer_bind 3797..3816 /label=VF2 primer primer_bind 208..227 /label=VR stem_loop 22..205 /label=B0025 terminator stem_loop 3895..3934 /label=B0010 terminator misc_feature 314..2350 /label=I50042 low copy pSC101 origin CDS 2600..3787 /label=Tetracycline resistance gene misc_feature 3252..3252 /label=c -> g BbsI site removed ORIGIN 1 tactagtagc ggccgctgca gtataaacgc agaaaggccc acccgaaggt gagccagtgt 61 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg 121 actgagcctt tcgttttatt tgatgcctgg gcttcctcgc tcactgactc gctgcgctcg 181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 301 cgtaaaaagg ccgctgtcag accaagttta cgagctcgct tggactcctg ttgatagatc 361 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt 421 tttttattgg tgagaatcca agcactaggg acagtaagac gggtaagcct gttgatgata 481 ccgctgcctt actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt 541 ggtcagactg gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca 601 catagcagac ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg 661 ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact 721 gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg 781 atggtcggag acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa 841 gcctttaggg ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt 901 gtaatactgc ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt 961 tatctttttt tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa 1021 aacacacaaa ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc 1081 aaaggtctag cagaatttac agatacccac aactcaaagg aaaaggacat gtaattatca 1141 ttgactagcc catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat 1201 gtctgaatta gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga 1261 gcatgaaacc aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc 1321 tacaaggaaa gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat 1381 cagtagggaa aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac 1441 tgtggaaatc aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc 1501 caagttctca agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt 1561 ccagttaaaa aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata 1621 ctctatgagg atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc 1681 aaatatagag attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca 1741 tgagtttaaa aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta 1801 cagcaatatg aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca 1861 agttgaacta gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat 1921 gaatggtgac aaaataccaa caaccattac atcagattcc tacctacata acggactaag 1981 aaaaacacta cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt 2041 tttgagtgac atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa 2101 acaacgaacc acactagaga acatactggc taaatacgga aggatctgag gttcttatgg 2161 ctcttgtatc tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc 2221 gttacatatc aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag 2281 atacatcaga gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg 2341 acaatgtaac tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 2401 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 2461 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc gagattctca 2521 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta 2581 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt 2641 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga 2701 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt 2761 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc 2821 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc 2881 cgtcctgtgg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc 2941 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg 3001 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt 3061 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct 3121 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt 3181 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 3241 acttatgact gtgttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 3301 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt 3361 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 3421 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct 3481 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg 3541 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg 3601 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat 3661 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg 3721 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac 3781 ctcgacctaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 3841 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatgatt tctgcgccgc 3901 aaaccccgcc cctgacaggg cggggtttcg ccgcgaattc gcggccgctt ctagaggcat 3961 acagtcgt //