##gff-version 3
##sequence-region BBa_J119051 1 3968
BBa_J119051	.	engineered_region	3935	3956	.	+	0	ID=annotation2169733;Name=Prefix
BBa_J119051	.	primer_binding_site	3797	3816	.	+	0	ID=annotation2169735;Name=VF2 primer
BBa_J119051	.	sequence_feature	3957	3968	.	+	0	ID=annotation2169734;Name=stuffer
BBa_J119051	.	primer_binding_site	208	227	.	+	0	ID=annotation2169736;Name=VR
BBa_J119051	.	stem_loop	22	205	.	+	0	ID=annotation2169737;Name=B0025 terminator
BBa_J119051	.	point_mutation	3252	3252	.	+	0	ID=annotation2169743;Name=c -> g BbsI site removed
BBa_J119051	.	CDS	2600	3787	.	+	0	ID=annotation2169740;Name=Tetracycline resistance gene
BBa_J119051	.	sequence_feature	314	2350	.	+	0	ID=annotation2169739;Name=I50042 low copy pSC101 origin
BBa_J119051	.	engineered_region	1	21	.	+	0	ID=annotation2169732;Name=Suffix
BBa_J119051	.	stem_loop	3895	3934	.	+	0	ID=annotation2169738;Name=B0010 terminator
>BBa_J119051
tactagtagcggccgctgcagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgtt
caccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctgggcttcctc
gctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatcc
acagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgctg
tcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttc
agaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttg
atgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaa
aatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaa
gcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttaccc
ccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggag
acaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgta
gaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctggg
atctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacac
aaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatac
ccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctaga
ccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcat
gaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtat
cgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccag
agagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgcc
aagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataa
aatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaac
acaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactac
catgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattgg
tggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccga
acttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacgga
ctaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgc
aaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaa
atacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaac
tgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagag
cttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaactcttttctacggggtctgac
gctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaa
attaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagattctcatgtttgac
agcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatc
taacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccg
ggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgc
aatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttgg
agccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatc
accggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggc
tcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgc
accattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataaggga
gagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccg
cacttatgactgtgttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccg
ctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtc
actggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtct
tgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgc
gttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagc
ctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatgga
ttgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaact
cgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatt
tcagataaaaaaaatgatttctgcgccgcaaaccccgcccctgacagggcggggtttcgccgcgaattcgcggccgctt
ctagaggcatacagtcgt