LOCUS       BBa_J119053              870 bp    DNA     linear       17-May-2024
DEFINITION  J23100 Promoter with BD18 RBS w/ leader and RFP reporter
ACCESSION   BBa_J119053
VERSION     BBa_J119053.1  
FEATURES             Location/Qualifiers
     misc_feature    1..22
                     /label=BBa_prefix
     misc_feature    17..20
                     /label=BbsI Word 1
     misc_feature    23..28
                     /label=BbsI cuts left
     misc_feature    29..32
                     /label=Bsa1 Word 1
     promoter        33..67
                     /label=J23100
     misc_feature    68..71
                     /label=BsaI Word 2
     misc_feature    72..77
                     /label=BbsI cuts right
     misc_feature    80..83
                     /label=BbsI Word 2
     CDS             84..171
                     /label=BD18 Bicistron
     misc_feature    101..109
                     /label=leader RBS
     misc_feature    154..162
                     /label=GOI RBS
     misc_feature    167..169
                     /label=stop for BD18
     misc_feature    169..171
                     /label=Start GOI
     CDS             169..849
                     /label=RFP E1010 with 4 pt mutations
     misc_feature    186..186
                     /label=C--> T for E. coli
     misc_feature    471..471
                     /label=C--> T for E. coli
     misc_feature    630..630
                     /label=C--> T for E. coli
     misc_feature    789..789
                     /label=C--> T for E. coli
     misc_feature    844..849
                     /label=Stop E1010
     misc_feature    850..870
                     /label=BBa_suffix
ORIGIN
        1 gaattcgcgg ccgcttctag aggtcttccg acttgacggc tagctcagtc ctaggtacag
       61 tgctagcgcg ggaagacaac taggggccca agttcactta aaaaggagat caacaatgaa
      121 agcaattttc gtactgaaac atcttaatca tgcgacggag cgtttctaat ggcttcctcc
      181 gaagatgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
      241 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
      301 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
      361 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
      421 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga tggtggtgtt
      481 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
      541 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
      601 gcttccaccg aacgtatgta cccggaagat ggtgctctga aaggtgaaat caaaatgcgt
      661 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
      721 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
      781 aacgaagatt acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
      841 gcttaataat actagtagcg gccgctgcag
//