##gff-version 3
##sequence-region BBa_J119053 1 870
BBa_J119053	.	sequence_feature	23	28	.	+	0	ID=annotation2169817;Name=BbsI cuts left
BBa_J119053	.	stop_codon	167	169	.	+	0	ID=annotation2169826;Name=stop for BD18
BBa_J119053	.	point_mutation	789	789	.	+	0	ID=annotation2169832;Name=C--> T for E. coli
BBa_J119053	.	sequence_feature	17	20	.	+	0	ID=annotation2169816;Name=BbsI Word 1
BBa_J119053	.	sequence_feature	154	162	.	+	0	ID=annotation2169825;Name=GOI RBS
BBa_J119053	.	point_mutation	630	630	.	+	0	ID=annotation2169831;Name=C--> T for E. coli
BBa_J119053	.	engineered_region	1	22	.	+	0	ID=annotation2169815;Name=BBa_prefix
BBa_J119053	.	promoter	33	67	.	+	0	ID=annotation2169819;Name=J23100
BBa_J119053	.	engineered_region	850	870	.	+	0	ID=annotation2169834;Name=BBa_suffix
BBa_J119053	.	sequence_feature	68	71	.	+	0	ID=annotation2169820;Name=BsaI Word 2
BBa_J119053	.	point_mutation	471	471	.	+	0	ID=annotation2169830;Name=C--> T for E. coli
BBa_J119053	.	CDS	169	849	.	+	0	ID=annotation2169828;Name=RFP E1010 with 4 pt mutations
BBa_J119053	.	start_codon	169	171	.	+	0	ID=annotation2169827;Name=Start GOI
BBa_J119053	.	sequence_feature	72	77	.	+	0	ID=annotation2169821;Name=BbsI cuts right
BBa_J119053	.	sequence_feature	101	109	.	+	0	ID=annotation2169824;Name=leader RBS
BBa_J119053	.	point_mutation	186	186	.	+	0	ID=annotation2169829;Name=C--> T for E. coli
BBa_J119053	.	CDS	84	171	.	+	0	ID=annotation2169823;Name=BD18 Bicistron
BBa_J119053	.	sequence_feature	80	83	.	+	0	ID=annotation2169822;Name=BbsI Word 2
BBa_J119053	.	sequence_feature	29	32	.	+	0	ID=annotation2169818;Name=Bsa1 Word 1
BBa_J119053	.	stop_codon	844	849	.	+	0	ID=annotation2169833;Name=Stop E1010
>BBa_J119053
gaattcgcggccgcttctagaggtcttccgacttgacggctagctcagtcctaggtacagtgctagcgcgggaagacaa
ctaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacgg
agcgtttctaatggcttcctccgaagatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaac
ggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttacca
aaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacaccc
ggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggt
ggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaact
tcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagatgg
tgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctac
atggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagatt
acaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagtagcggccgctgcag
g