LOCUS       BBa_J119054             3590 bp    DNA     linear       26-Nov-2024
DEFINITION  pLacI-RBS-T7 RNAP + RFPrev-RBSrev-pT7rev
ACCESSION   BBa_J119054
VERSION     BBa_J119054.1  
FEATURES             Location/Qualifiers
     promoter        1..56
                     /label=pLacI
     RBS             68..82
                     /label=B0030
     CDS             89..2810
                     /label=T7 RNAP
     misc_feature    115..126
                     /label=22 nt frameshift mutation
     CDS             1..2827
                     /label=BBa_J119057
     misc_feature    2836..2850
                     /label=Barcode
     misc_feature    2856..3540
                     /label=mRFP1rev
     stem_loop       3547..3559
                     /label=RBS34rev
     promoter        3567..3590
                     /label=pTetrev
     misc_feature    2836..3590
                     /label=BBa_J119018
ORIGIN
        1 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggcgtc
       61 tcggcgaatt aaagaggaga aagtaggcat gaacacgaga gaccacgcga tcgcgaggac
      121 gctaccccac cgctagcagg cgcgccaggt ctctgctgga agacgtcacg attaacatcg
      181 ctaagaacga cttctctgac atcgaactgg ctgctatccc gttcaacact ctagctgacc
      241 attacggtga gcgtttagct cgcgaacagt tggcccttga gcatgagtct tacgagatgg
      301 gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa agctggtgag gttgcggata
      361 acgctgccgc caagcctctc atcactaccc tactccctaa gatgattgca cgcatcaacg
      421 actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc gacagccttc cagttcctgc
      481 aagaaatcaa gccggaagcc gtagcgtaca tcaccattaa gaccactcta gcttgcctaa
      541 ccagtgctga caatacaacc gttcaggctg tagcaagcgc aatcggtcgg gccattgagg
      601 acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa gcacttcaag aaaaacgtcg
      661 aggaacaact caacaagcgc gtagggcacg tctacaagaa agcatttatg caagttgtcg
      721 aggctgacat gctctctaag ggtctactcg gtggcgaggc gtggtcctcg tggcataagg
      781 aggactctat tcatgtagga gtacgctgca tcgagatgct cattgagtca accggaatgg
      841 ttagcttaca ccgccaaaat gctggcgtag taggtcaaga ctctgagact atcgaactcg
      901 cacctgaata cgctgaggct atcgcaaccc gtgcaggggc gctggctggc atctctccga
      961 tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg cattactggt ggtggctatt
     1021 gggctaacgg tcgtcgtcct ctagcgctgg tgcgtactca cagtaagaaa gcactgatgc
     1081 gctacgagga cgtttacatg cctgaggtgt acaaagcgat taacattgcg caaaacaccg
     1141 catggaaaat caacaagaaa gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt
     1201 gtccggtcga ggacatccct gcgattgagc gtgaagaact cccgatgaaa ccggaggaca
     1261 tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca
     1321 aggacaaggc tcgcaagtct cgccgtatca gccttgagtt catgcttgag caagccaata
     1381 agtttgctaa ccataaggcc atctggttcc cttacaacat ggactggcgc ggtcgtgttt
     1441 acgctgtgtc aatgttcaac ccgcaaggta acgatatgac caaaggactg cttacgctgg
     1501 cgaaaggtaa accaatcggt aaggaaggtt actactggct gaaaatccac ggtgcaaact
     1561 gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa gttcattgag gaaaaccacg
     1621 agaacatcat ggcttgcgct aagtctccac tggagaacac ttggtgggct gagcaagatt
     1681 ctccgttctg cttccttgcg ttctgctttg agtacgctgg ggtacagcac cacggcctga
     1741 gctataactg ctcccttccg ctggcgtttg acgggtcttg ctccggcatc cagcacttct
     1801 ccgcgatgct ccgagatgag gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg
     1861 ttcaggacat ctacgggatt gttgctaaga aagtcaacga gattctacaa gcagacgcaa
     1921 tcaatgggac cgataacgaa gtagttaccg tgaccgatga gaacactggt gaaatctctg
     1981 agaaagtcaa gctgggcact aaggcactgg ctggtcaatg gctggcttac ggtgttactc
     2041 gcagtgtgac taagcgttca gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc
     2101 gtcaacaagt gctggaagat accattcagc cagctattga ttccggcaag ggtctgatgt
     2161 tcactcagcc gaatcaggct gctggataca tggctaagct gatttgggaa tctgtgagcg
     2221 tgacggtggt agctgcggtt gaagcaatga actggcttaa gtctgctgct aagctgctgg
     2281 ctgctgaggt caaagataag aaaactggag agattcttcg caagcgttgc gctgtgcatt
     2341 gggtaactcc tgatggtttc cctgtgtggc aggaatacaa gaagcctatt cagacgcgct
     2401 tgaacctgat gttcctcggt cagttccgct tacagcctac cattaacacc aacaaagata
     2461 gcgagattga tgcacacaaa caggagtctg gtatcgctcc taactttgta cacagccaag
     2521 acggtagcca ccttcgtaag actgtagtgt gggcacacga gaagtacgga atcgaatctt
     2581 ttgcactgat tcacgactcc ttcggtacca ttccggctga cgctgcgaac ctgttcaaag
     2641 cagtgcgcga aactatggtt gacacatatg agtcttgtga tgtactggct gatttctacg
     2701 accagttcgc tgaccagttg cacgagtctc aattggacaa aatgccagca cttccggcta
     2761 aaggtaactt gaacctccgt gacatcttag agtcggactt cgcgttcgcg taataacgca
     2821 tgagacgtac tagaggcgat ctacactagc actatcagcg ttattaagca ccggtggagt
     2881 gacgaccttc agcacgttcg tactgttcaa cgatggtgta gtcttcgttg tgggaggtga
     2941 tgtccagttt gatgtcggtt ttgtaagcac ccggcagctg aaccggtttt ttagccatgt
     3001 aggtggtttt aacttcagcg tcgtagtgac caccgtcttt cagtttcaga cgcattttga
     3061 tttcaccttt cagagcaccg tcttccgggt acatacgttc ggtggaagct tcccaaccca
     3121 tggttttttt ctgcataacc ggaccgtcgg acgggaagtt ggtaccacgc agtttaactt
     3181 tgtagatgaa ctcaccgtct tgcagggagg agtcctgggt aacggtaaca acaccaccgt
     3241 cttcgaagtt cataacacgt tcccatttga aaccttccgg gaaggacagt ttcaggtagt
     3301 ccgggatgtc agccgggtgt ttaacgtaag ctttggaacc gtactggaac tgcggggaca
     3361 ggatgtccca agcgaacggc agcggaccac ctttggtaac tttcagttta gcggtctggg
     3421 taccttcgta cggacgacct tcaccttcac cttcgatttc gaactcgtga ccgttaacgg
     3481 aaccttccat acgaactttg aaacgcatga actctttgat aacgtcttcg gaggaagcca
     3541 tctagtattt ctcctctttc tctagtatct ccctatagtg agtcgtatta
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