##gff-version 3
##sequence-region BBa_J119054 1 3590
BBa_J119054	.	engineered_region	2836	3590	.	+	0	ID=BBa_J119018;Name=BBa_J119018
BBa_J119054	.	stem_loop	3547	3559	.	+	0	ID=annotation2158196;Name=RBS34rev;Parent=BBa_J119018
BBa_J119054	.	promoter	3567	3590	.	+	0	ID=annotation2158197;Name=pTetrev;Parent=BBa_J119018
BBa_J119054	.	sequence_feature	2856	3540	.	+	0	ID=annotation2158195;Name=mRFP1rev;Parent=BBa_J119018
BBa_J119054	.	sequence_feature	2836	2850	.	+	0	ID=annotation2158194;Name=Barcode;Parent=BBa_J119018
BBa_J119054	.	CDS	1	2827	.	+	0	ID=BBa_J119057;Name=BBa_J119057
BBa_J119054	.	sequence_alteration	115	126	.	+	0	ID=annotation2169868;Name=22 nt frameshift mutation;Parent=BBa_J119057
BBa_J119054	.	CDS	89	2810	.	+	0	ID=annotation2169867;Name=T7 RNAP;Parent=BBa_J119057
BBa_J119054	.	ribosome_entry_site	68	82	.	+	0	ID=annotation2169866;Name=B0030;Parent=BBa_J119057
BBa_J119054	.	promoter	1	56	.	+	0	ID=annotation2169865;Name=pLacI;Parent=BBa_J119057
>BBa_J119054
cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggag
aaagtaggcatgaacacgagagaccacgcgatcgcgaggacgctaccccaccgctagcaggcgcgccaggtctctgctg
gaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctg
accattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccg
caagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactc
cctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagt
tcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaa
tacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgacctt
gaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgc
aagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctat
tcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgta
gtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctg
gcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaa
cggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgag
gtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatca
ccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcga
catgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgc
cgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatgg
actggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggc
gaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggtt
ccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaaca
cttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcct
gagctataactgctcccttccgctggcgtttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagat
gaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtca
acgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaat
ctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaag
cgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccag
ctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatc
tgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtc
aaagataagaaaactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggc
aggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacac
caacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagc
caccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggta
ccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtact
ggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggt
aacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataacgcatgagacgtactagaggcgatctac
actagcactatcagcgttattaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtc
ttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtag
gtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgt
cttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagtt
ggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccg
tcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggt
gtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggt
aactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgtta
acggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccatctagtatttctcct
ctttctctagtatctccctatagtgagtcgtatta