LOCUS BBa_J119062 3742 bp DNA linear 22-Nov-2024 DEFINITION pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 1) ACCESSION BBa_J119062 VERSION BBa_J119062.1 FEATURES Location/Qualifiers misc_feature 18..18 /label=Transcription Start Site misc_feature 1..11 /label=Polymerase Binding Region misc_feature 12..23 /label=Initiation Region promoter 1..23 /label=BBa_R0085 RBS 32..44 /label=RBS-3\Weak RBS 38..41 /label=RBS misc_feature 32..44 /label=BBa_B0032 RBS 32..44 /label=BBa_B0032 CDS 51..770 /label=GFP protein CDS 51..770 /label=BBa_E0040 stem_loop 790..833 /label=stem_loop misc_feature 779..858 /label=BBa_B0010 terminator 779..858 /label=BBa_B0010 stem_loop 874..893 /label=T7 TE misc_feature 900..900 /label=stop polyA_site 894..907 /label=polya misc_feature 867..907 /label=BBa_B0012 terminator 867..907 /label=BBa_B0012 misc_feature 1..907 /label=BBa_K199150 promoter 916..971 /label=pLacI RBS 983..997 /label=B0030 CDS 1004..3725 /label=T7 RNAP misc_feature 1030..1041 /label=22 nt frameshift insertion CDS 916..3742 /label=BBa_J119055 ORIGIN 1 taatacgact cactataggg agatactaga gtcacacagg aaagtactag atgcgtaaag 61 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg 121 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc 181 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt 241 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt 301 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg 361 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 421 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca 481 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta 541 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac 601 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac 661 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 721 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc 781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 841 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 901 gtttatatac tagagcgttg acaccatcga atggcgcaaa acctttcgcg gtatggcatg 961 atagcgcccg gcgtctcggc gaattaaaga ggagaaagta ggcatgaaca cgagagacca 1021 cgcgatcgcg ccaccgagga cccaatgcta ctaggcgcgc caggtctctg ctggaagacg 1081 tcacgattaa catcgctaag aacgacttct ctgacatcga actggctgct atcccgttca 1141 acactctagc tgaccattac ggtgagcgtt tagctcgcga acagttggcc cttgagcatg 1201 agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg 1261 gtgaggttgc ggataacgct gccgccaagc ctctcatcac taccctactc cctaagatga 1321 ttgcacgcat caacgactgg tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag 1381 ccttccagtt cctgcaagaa atcaagccgg aagccgtagc gtacatcacc attaagacca 1441 ctctagcttg cctaaccagt gctgacaata caaccgttca ggctgtagca agcgcaatcg 1501 gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact 1561 tcaagaaaaa cgtcgaggaa caactcaaca agcgcgtagg gcacgtctac aagaaagcat 1621 ttatgcaagt tgtcgaggct gacatgctct ctaagggtct actcggtggc gaggcgtggt 1681 cctcgtggca taaggaggac tctattcatg taggagtacg ctgcatcgag atgctcattg 1741 agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt caagactctg 1801 agactatcga actcgcacct gaatacgctg aggctatcgc aacccgtgca ggggcgctgg 1861 ctggcatctc tccgatgttc caaccttgcg tagttcctcc taagccgtgg actggcatta 1921 ctggtggtgg ctattgggct aacggtcgtc gtcctctagc gctggtgcgt actcacagta 1981 agaaagcact gatgcgctac gaggacgttt acatgcctga ggtgtacaaa gcgattaaca 2041 ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca 2101 ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga 2161 tgaaaccgga ggacatcgac atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg 2221 ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg tatcagcctt gagttcatgc 2281 ttgagcaagc caataagttt gctaaccata aggccatctg gttcccttac aacatggact 2341 ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag 2401 gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga aggttactac tggctgaaaa 2461 tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc atcaagttca 2521 ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc tccactggag aacacttggt 2581 gggctgagca agattctccg ttctgcttcc ttgcgttctg ctttgagtac gctggggtac 2641 agcaccacgg cctgagctat aactgctccc ttccgctggc gtttgacggg tcttgctccg 2701 gcatccagca cttctccgcg atgctccgag atgaggtagg tggtcgcgcg gttaacttgc 2761 ttcctagtga aaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc 2821 tacaagcaga cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca 2881 ctggtgaaat ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg 2941 cttacggtgt tactcgcagt gtgactaagc gttcagtcat gacgctggct tacgggtcca 3001 aagagttcgg cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg 3061 gcaagggtct gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt 3121 gggaatctgt gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg 3181 ctgctaagct gctggctgct gaggtcaaag ataagaaaac tggagagatt cttcgcaagc 3241 gttgcgctgt gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc 3301 ctattcagac gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta 3361 acaccaacaa agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact 3421 ttgtacacag ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt 3481 acggaatcga atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg 3541 cgaacctgtt caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac 3601 tggctgattt ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc 3661 cagcacttcc ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt 3721 tcgcgtaata acgcatgaga cg //