##gff-version 3
##sequence-region BBa_J119064 1 3742
BBa_J119064	.	engineered_region	1	907	.	+	0	ID=BBa_K199150;Name=BBa_K199150
BBa_J119064	.	promoter	1	23	.	+	0	ID=BBa_R0085;Name=BBa_R0085;Parent=BBa_K199150
BBa_J119064	.	non_covalent_binding_site	1	11	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_J119064	.	sequence_feature	12	23	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_J119064	.	sequence_feature	18	18	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
BBa_J119064	.	CDS	51	770	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K199150
BBa_J119064	.	CDS	51	770	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J119064	.	terminator	779	858	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K199150
BBa_J119064	.	engineered_region	779	858	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J119064	.	stem_loop	790	833	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J119064	.	terminator	867	907	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K199150
BBa_J119064	.	stop_codon	900	900	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J119064	.	polyA_site	894	907	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J119064	.	stem_loop	874	893	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J119064	.	engineered_region	867	907	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J119064	.	ribosome_entry_site	32	44	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K199150
BBa_J119064	.	ribosome_entry_site	32	44	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J119064	.	engineered_region	32	44	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J119064	.	ribosome_entry_site	38	41	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J119064	.	CDS	916	3742	.	+	0	ID=BBa_J119057;Name=BBa_J119057
BBa_J119064	.	CDS	1004	3725	.	+	0	ID=annotation2169867;Name=T7 RNAP;Parent=BBa_J119057
BBa_J119064	.	ribosome_entry_site	983	997	.	+	0	ID=annotation2169866;Name=B0030;Parent=BBa_J119057
BBa_J119064	.	sequence_alteration	1030	1041	.	+	0	ID=annotation2169868;Name=22 nt frameshift mutation;Parent=BBa_J119057
BBa_J119064	.	promoter	916	971	.	+	0	ID=annotation2169865;Name=pLacI;Parent=BBa_J119057
>BBa_J119064
taatacgactcactatagggagatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactgg
agttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgat
gcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcc
cgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaa
ggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattgg
aatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattag
acacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctt
ttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttc
ttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagcgttgacaccatcgaatggcgcaaaacctttcg
cggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgcgatc
gcgaggacgctaccccaccgctagcaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgac
ttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagt
tggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtga
ggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggttt
gaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgt
acatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcgg
tcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaa
caactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtc
tactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcat
tgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgca
cctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttc
ctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactca
cagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacacc
gcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatcc
ctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtggaaacg
tgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagcc
aataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgt
tcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttacta
ctggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaa
aaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgct
tccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttga
cgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcct
agtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatggga
ccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcact
ggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaa
gagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactc
agccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagc
aatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaaaactggagagattcttcgcaag
cgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttga
acctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaaca
ggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgag
aagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaag
cagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagtt
gcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggac
ttcgcgttcgcgtaataacgcatgagacg