BBa_J119067
type: ComponentDefinition
persistent identity: BBa J119067
display id: BBa_J119067
version: 1
was generated by: iGEM to SBOL conversion
description: pT7-RBS-GFP-TT-pLacI-RBS-T7 RNAP (mutant 6)
created: 2012-01-31T12:00:00Z
modified: 2015-08-31T04:08:26Z
mutable provenance: MWSU and Davidson College
owned by: james
owned by: myers
mutable description: This construct consists of a GFP reporter with a T7 promoter. The T7RNAP has a frameshift mutation inserted at the beginning of the gene. The mutation consists of an 11 base oligonucleotide.
discontinued: false
dominant: false
experience: experience/None
group u list: _613_
m user id: 0
owner id: 10415
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Jerrad Morton
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K199150
component: BBa_J119060
sequence annotation: BBa_K199150
sequence annotation: BBa_J119060
sequence: https://synbiohub.org/public/igem/BBa_J119067_sequence/1

BBa_K199150
type: Component
persistent identity: component2170009
display id: component2170009
version: 1
top level: BBa_J119067
definition: BBa_K199150
access: public

BBa_J119060
type: Component
persistent identity: component2170014
display id: component2170014
version: 1
top level: BBa_J119067
definition: BBa_J119060
access: public

BBa_K199150
type: SequenceAnnotation
persistent identity: annotation2170009
display id: annotation2170009
version: 1
top level: BBa_J119067
location: https://synbiohub.org/public/igem/BBa_J119067/annotation2170009/range2170009/1
component: BBa_K199150

https://synbiohub.org/public/igem/BBa_J119067/annotation2170009/range2170009/1
type: Range
persistent identity: range2170009
display id: range2170009
version: 1
top level: BBa_J119067
direction: direction/reverse
start: 1
end: 907
orientation: inline

BBa_J119060
type: SequenceAnnotation
persistent identity: annotation2170014
display id: annotation2170014
version: 1
top level: BBa_J119067
location: https://synbiohub.org/public/igem/BBa_J119067/annotation2170014/range2170014/1
component: BBa_J119060

https://synbiohub.org/public/igem/BBa_J119067/annotation2170014/range2170014/1
type: Range
persistent identity: range2170014
display id: range2170014
version: 1
top level: BBa_J119067
direction: direction/reverse
start: 916
end: 3742
orientation: inline

BBa_K199150
type: ComponentDefinition
persistent identity: BBa K199150
display id: BBa_K199150
version: 1
was generated by: iGEM to SBOL conversion
description: pT7-RBS-GFP-TT
created: 2010-05-04T11:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: Biobrick parts
owned by: james
owned by: myers
mutable description: pT7-RBS-GFP-TT
discontinued: false
dominant: true
group u list: _295_
m user id: 0
owner id: 5118
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Clifton Davis
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0085
component: BBa_E0040
component: BBa_B0032
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0085
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K199150_sequence/1

BBa_R0085
type: Component
persistent identity: component2068776
display id: component2068776
version: 1
top level: BBa_K199150
definition: BBa_R0085
access: public

BBa_E0040
type: Component
persistent identity: component2068781
display id: component2068781
version: 1
top level: BBa_K199150
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component2068778
display id: component2068778
version: 1
top level: BBa_K199150
definition: BBa_B0032
access: public

BBa_B0012
type: Component
persistent identity: component2068784
display id: component2068784
version: 1
top level: BBa_K199150
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2068782
display id: component2068782
version: 1
top level: BBa_K199150
definition: BBa_B0010
access: public

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation2068776
display id: annotation2068776
version: 1
top level: BBa_K199150
location: https://synbiohub.org/public/igem/BBa_K199150/annotation2068776/range2068776/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_K199150/annotation2068776/range2068776/1
type: Range
persistent identity: range2068776
display id: range2068776
version: 1
top level: BBa_K199150
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2068778
display id: annotation2068778
version: 1
top level: BBa_K199150
location: https://synbiohub.org/public/igem/BBa_K199150/annotation2068778/range2068778/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K199150/annotation2068778/range2068778/1
type: Range
persistent identity: range2068778
display id: range2068778
version: 1
top level: BBa_K199150
direction: direction/forward
start: 32
end: 44
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2068781
display id: annotation2068781
version: 1
top level: BBa_K199150
location: https://synbiohub.org/public/igem/BBa_K199150/annotation2068781/range2068781/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K199150/annotation2068781/range2068781/1
type: Range
persistent identity: range2068781
display id: range2068781
version: 1
top level: BBa_K199150
direction: direction/reverse
start: 51
end: 770
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2068782
display id: annotation2068782
version: 1
top level: BBa_K199150
location: https://synbiohub.org/public/igem/BBa_K199150/annotation2068782/range2068782/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K199150/annotation2068782/range2068782/1
type: Range
persistent identity: range2068782
display id: range2068782
version: 1
top level: BBa_K199150
direction: direction/forward
start: 779
end: 858
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2068784
display id: annotation2068784
version: 1
top level: BBa_K199150
location: https://synbiohub.org/public/igem/BBa_K199150/annotation2068784/range2068784/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K199150/annotation2068784/range2068784/1
type: Range
persistent identity: range2068784
display id: range2068784
version: 1
top level: BBa_K199150
direction: direction/forward
start: 867
end: 907
orientation: inline

BBa_J119060
type: ComponentDefinition
persistent identity: BBa J119060
display id: BBa_J119060
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI-RBS-T7 RNAP (2SAT mutant 6)
created: 2012-01-26T12:00:00Z
modified: 2015-08-31T04:08:26Z
mutable provenance: Existing part and synthetic oligos.
owned by: james
owned by: myers
mutable description: T7 RNAP gene has frameshift mutation inserted that prevents it from being expressed and causing RFP expression. Suppression by 5 nt anticodon tRNAs is expected to rescue the RFP phenotype. The frameshift mutation is of the form (a??? or b) and (a or c???).
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The frameshift mutation is of the form (a??? or b) and (a or c???).
star: false
creator: Jerrad Morton
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pLacI
sequence annotation: B0030
sequence annotation: T7 RNAP
sequence annotation: 22 nt frameshift mutation
sequence: https://synbiohub.org/public/igem/BBa_J119060_sequence/1

pLacI
type: SequenceAnnotation
persistent identity: annotation2169877
display id: annotation2169877
version: 1
top level: BBa_J119060
location: https://synbiohub.org/public/igem/BBa_J119060/annotation2169877/range2169877/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J119060/annotation2169877/range2169877/1
type: Range
persistent identity: range2169877
display id: range2169877
version: 1
top level: BBa_J119060
direction: direction/forward
start: 1
end: 56
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation2169878
display id: annotation2169878
version: 1
top level: BBa_J119060
location: https://synbiohub.org/public/igem/BBa_J119060/annotation2169878/range2169878/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J119060/annotation2169878/range2169878/1
type: Range
persistent identity: range2169878
display id: range2169878
version: 1
top level: BBa_J119060
direction: direction/forward
start: 68
end: 82
orientation: inline

T7 RNAP
type: SequenceAnnotation
persistent identity: annotation2169879
display id: annotation2169879
version: 1
top level: BBa_J119060
location: https://synbiohub.org/public/igem/BBa_J119060/annotation2169879/range2169879/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J119060/annotation2169879/range2169879/1
type: Range
persistent identity: range2169879
display id: range2169879
version: 1
top level: BBa_J119060
direction: direction/forward
start: 89
end: 2810
orientation: inline

22 nt frameshift mutation
type: SequenceAnnotation
persistent identity: annotation2169880
display id: annotation2169880
version: 1
top level: BBa_J119060
location: https://synbiohub.org/public/igem/BBa_J119060/annotation2169880/range2169880/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J119060/annotation2169880/range2169880/1
type: Range
persistent identity: range2169880
display id: range2169880
version: 1
top level: BBa_J119060
direction: direction/forward
start: 115
end: 126
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

https://synbiohub.org/public/igem/BBa_J119067_sequence/1
type: Sequence
persistent identity: BBa J119067 sequence
display id: BBa_J119067_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgcgatcgcgccaccgctactctagcgctaccaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactgatgcgctac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199150_sequence/1
type: Sequence
persistent identity: BBa K199150 sequence
display id: BBa_K199150_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J119060_sequence/1
type: Sequence
persistent identity: BBa J119060 sequence
display id: BBa_J119060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgcgatcgcgccaccgctactctagcgctaccaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z