LOCUS BBa_J119068 3590 bp DNA linear 22-Nov-2024 DEFINITION RFPrev-RBSrev-pT7rev-pLacI-RBS-T7 RNAP (2SAT mutant 1) ACCESSION BBa_J119068 VERSION BBa_J119068.1 FEATURES Location/Qualifiers misc_feature 1..15 /label=Barcode misc_feature 21..705 /label=mRFP1rev stem_loop 712..724 /label=RBS34rev promoter 732..755 /label=pTetrev misc_feature 1..755 /label=BBa_J119018 promoter 764..819 /label=pLacI RBS 831..845 /label=B0030 CDS 852..3573 /label=T7 RNAP misc_feature 878..889 /label=22 nt frameshift insertion CDS 764..3590 /label=BBa_J119055 ORIGIN 1 gcgatctaca ctagcactat cagcgttatt aagcaccggt ggagtgacga ccttcagcac 61 gttcgtactg ttcaacgatg gtgtagtctt cgttgtggga ggtgatgtcc agtttgatgt 121 cggttttgta agcacccggc agctgaaccg gttttttagc catgtaggtg gttttaactt 181 cagcgtcgta gtgaccaccg tctttcagtt tcagacgcat tttgatttca cctttcagag 241 caccgtcttc cgggtacata cgttcggtgg aagcttccca acccatggtt tttttctgca 301 taaccggacc gtcggacggg aagttggtac cacgcagttt aactttgtag atgaactcac 361 cgtcttgcag ggaggagtcc tgggtaacgg taacaacacc accgtcttcg aagttcataa 421 cacgttccca tttgaaacct tccgggaagg acagtttcag gtagtccggg atgtcagccg 481 ggtgtttaac gtaagctttg gaaccgtact ggaactgcgg ggacaggatg tcccaagcga 541 acggcagcgg accacctttg gtaactttca gtttagcggt ctgggtacct tcgtacggac 601 gaccttcacc ttcaccttcg atttcgaact cgtgaccgtt aacggaacct tccatacgaa 661 ctttgaaacg catgaactct ttgataacgt cttcggagga agccatctag tatttctcct 721 ctttctctag tatctcccta tagtgagtcg tattatacta gagcgttgac accatcgaat 781 ggcgcaaaac ctttcgcggt atggcatgat agcgcccggc gtctcggcga attaaagagg 841 agaaagtagg catgaacacg agagaccacg cgatcgcgcc accgaggacc caatgctact 901 aggcgcgcca ggtctctgct ggaagacgtc acgattaaca tcgctaagaa cgacttctct 961 gacatcgaac tggctgctat cccgttcaac actctagctg accattacgg tgagcgttta 1021 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc 1081 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct 1141 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg 1201 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa 1261 gccgtagcgt acatcaccat taagaccact ctagcttgcc taaccagtgc tgacaataca 1321 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt 1381 cgtatccgtg accttgaagc taagcacttc aagaaaaacg tcgaggaaca actcaacaag 1441 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct 1501 aagggtctac tcggtggcga ggcgtggtcc tcgtggcata aggaggactc tattcatgta 1561 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa 1621 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag 1681 gctatcgcaa cccgtgcagg ggcgctggct ggcatctctc cgatgttcca accttgcgta 1741 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt 1801 cctctagcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga ggacgtttac 1861 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag 1921 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc 1981 cctgcgattg agcgtgaaga actcccgatg aaaccggagg acatcgacat gaatcctgag 2041 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag 2101 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag 2161 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc 2221 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc 2281 ggtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag 2341 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc 2401 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt 2461 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt 2521 ccgctggcgt ttgacgggtc ttgctccggc atccagcact tctccgcgat gctccgagat 2581 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg 2641 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac 2701 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc 2761 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt 2821 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa 2881 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag 2941 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg 3001 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat 3061 aagaaaactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt 3121 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc 3181 ggtcagttcc gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac 3241 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt 3301 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac 3361 tccttcggta ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg 3421 gttgacacat atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag 3481 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc 3541 cgtgacatct tagagtcgga cttcgcgttc gcgtaataac gcatgagacg //