LOCUS       BBa_J119070             3590 bp    DNA     linear       22-Dec-2024
DEFINITION  pT7-RBS-RFP-pLacI-RBS-T7RNAP (mutant 2)
ACCESSION   BBa_J119070
VERSION     BBa_J119070.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..724
                     /label=mrfp1
     misc_feature    731..755
                     /label=Help:Barcodes
     CDS             50..755
                     /label=BBa_E1010
     misc_feature    1..755
                     /label=BBa_K199118
     promoter        764..819
                     /label=pLacI
     RBS             831..845
                     /label=B0030
     CDS             852..3573
                     /label=T7 RNAP
     misc_feature    878..889
                     /label=22 nt frameshift mutation
     CDS             764..3590
                     /label=BBa_J119056
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      541 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      601 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      661 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      721 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagcgttgac accatcgaat
      781 ggcgcaaaac ctttcgcggt atggcatgat agcgcccggc gtctcggcga attaaagagg
      841 agaaagtagg catgaacacg agagaccacg cgatcgcgct agcgctactc caatgaggac
      901 aggcgcgcca ggtctctgct ggaagacgtc acgattaaca tcgctaagaa cgacttctct
      961 gacatcgaac tggctgctat cccgttcaac actctagctg accattacgg tgagcgttta
     1021 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc
     1081 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct
     1141 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg
     1201 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa
     1261 gccgtagcgt acatcaccat taagaccact ctagcttgcc taaccagtgc tgacaataca
     1321 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt
     1381 cgtatccgtg accttgaagc taagcacttc aagaaaaacg tcgaggaaca actcaacaag
     1441 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct
     1501 aagggtctac tcggtggcga ggcgtggtcc tcgtggcata aggaggactc tattcatgta
     1561 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa
     1621 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag
     1681 gctatcgcaa cccgtgcagg ggcgctggct ggcatctctc cgatgttcca accttgcgta
     1741 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt
     1801 cctctagcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga ggacgtttac
     1861 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag
     1921 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc
     1981 cctgcgattg agcgtgaaga actcccgatg aaaccggagg acatcgacat gaatcctgag
     2041 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag
     2101 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag
     2161 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc
     2221 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc
     2281 ggtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag
     2341 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc
     2401 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt
     2461 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt
     2521 ccgctggcgt ttgacgggtc ttgctccggc atccagcact tctccgcgat gctccgagat
     2581 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg
     2641 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac
     2701 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc
     2761 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt
     2821 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa
     2881 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag
     2941 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg
     3001 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat
     3061 aagaaaactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt
     3121 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc
     3181 ggtcagttcc gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac
     3241 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt
     3301 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac
     3361 tccttcggta ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg
     3421 gttgacacat atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag
     3481 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc
     3541 cgtgacatct tagagtcgga cttcgcgttc gcgtaataac gcatgagacg
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