##gff-version 3
##sequence-region BBa_J119074 1 3590
BBa_J119074	.	CDS	764	3590	.	+	0	ID=BBa_J119060;Name=BBa_J119060
BBa_J119074	.	promoter	764	819	.	+	0	ID=annotation2169877;Name=pLacI;Parent=BBa_J119060
BBa_J119074	.	ribosome_entry_site	831	845	.	+	0	ID=annotation2169878;Name=B0030;Parent=BBa_J119060
BBa_J119074	.	sequence_alteration	878	889	.	+	0	ID=annotation2169880;Name=22 nt frameshift mutation;Parent=BBa_J119060
BBa_J119074	.	CDS	852	3573	.	+	0	ID=annotation2169879;Name=T7 RNAP;Parent=BBa_J119060
BBa_J119074	.	engineered_region	1	755	.	+	0	ID=BBa_K199118;Name=BBa_K199118
BBa_J119074	.	CDS	50	755	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K199118
BBa_J119074	.	CDS	50	724	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J119074	.	engineered_tag	731	755	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J119074	.	ribosome_entry_site	32	43	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199118
BBa_J119074	.	conserved_region	36	39	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J119074	.	promoter	1	23	.	+	0	ID=BBa_R0085;Name=BBa_R0085;Parent=BBa_K199118
BBa_J119074	.	non_covalent_binding_site	1	11	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_J119074	.	sequence_feature	18	18	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
BBa_J119074	.	sequence_feature	12	23	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
>BBa_J119074
taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagag
ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc
cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc
gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa
ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg
gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg
ttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaa
gacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaa
ccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca
ctccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcgttgacaccatcgaatggcgcaaaac
ctttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccac
gcgatcgcgccaccgctactctagcgctaccaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaag
aacgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcg
aacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagc
tggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgac
tggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccg
tagcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgc
aatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtc
gaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctcta
agggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagat
gctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaa
ctcgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcg
tagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcg
tactcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaa
aacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgagg
acatccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtg
gaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgag
caagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgt
caatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaagg
ttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcatt
gaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgt
tctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggc
gtttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttg
cttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatca
atgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaa
ggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacggg
tccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgt
tcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggt
tgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaaaactggagagattctt
cgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgc
gcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcaca
caaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggca
cacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgt
tcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctga
ccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagag
tcggacttcgcgttcgcgtaataacgcatgagacg