LOCUS BBa_J119078 3904 bp DNA linear 08-Dec-2025 DEFINITION tRNA-pLacI-RBS-T7RNAP (mutant 6 and tRNA 91) ACCESSION BBa_J119078 VERSION BBa_J119078.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 254..258 /label=5nt anti codon misc_feature 209..351 /label=BBa_K199048 CDS 360..447 /label=end of LacI coding region (inactive) misc_feature 360..559 /label=BBa_R0010 misc_feature 448..485 /label=CAP binding site promoter 496..501 /label=-35 promoter 520..525 /label=-10 misc_feature 525..559 /label=LacI binding site misc_feature 532..532 /label=start promoter 360..559 /label=BBa_R0010 misc_feature 613..617 /label=5 nt anticodon misc_feature 568..710 /label=BBa_K199014 CDS 719..806 /label=end of LacI coding region (inactive) misc_feature 719..918 /label=BBa_R0010 misc_feature 807..844 /label=CAP binding site promoter 855..860 /label=-35 promoter 879..884 /label=-10 misc_feature 884..918 /label=LacI binding site misc_feature 891..891 /label=start promoter 719..918 /label=BBa_R0010 misc_feature 972..976 /label=5nt anti codon misc_feature 927..1069 /label=BBa_K199045 misc_feature 1..1069 /label=BBa_K199191 promoter 1078..1133 /label=pLacI RBS 1145..1159 /label=B0030 CDS 1166..3887 /label=T7 RNAP misc_feature 1192..1203 /label=22 nt frameshift mutation CDS 1078..3904 /label=BBa_J119060 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagaggg atccaattcg gagagatgcc ggagcggctg 241 aacggaccgg tttggtggaa accggagtag gggcaactct accgggggtt caaatccccc 301 tctctccgcc actgcatatc cttagcgaaa gctaaggatt ttttttaagc ttactagagc 361 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 421 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 481 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 541 cggataacaa tttcacacat actagaggga tccaattcgg agagatgccg gagcggctga 601 acggaccggt ctgtcctaaa ccggagtagg ggcaactcta ccgggggttc aaatccccct 661 ctctccgcca ctgcatatcc ttagcgaaag ctaaggattt tttttaagct tactagagca 721 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 781 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 841 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 901 ggataacaat ttcacacata ctagagggat ccaattcgga gagatgccgg agcggctgaa 961 cggaccggtg tggtagaaac cggagtaggg gcaactctac cgggggttca aatccccctc 1021 tctccgccac tgcatatcct tagcgaaagc taaggatttt ttttaagctt actagagcgt 1081 tgacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggcgtctcg 1141 gcgaattaaa gaggagaaag taggcatgaa cacgagagac cacgcgatcg cgccaccgct 1201 actctagcgc taccaggcgc gccaggtctc tgctggaaga cgtcacgatt aacatcgcta 1261 agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactcta gctgaccatt 1321 acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg 1381 aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg 1441 ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact 1501 ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgcaag 1561 aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctagct tgcctaacca 1621 gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg 1681 aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgtcgagg 1741 aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg 1801 ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcctcgtgg cataaggagg 1861 actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta 1921 gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac 1981 ctgaatacgc tgaggctatc gcaacccgtg caggggcgct ggctggcatc tctccgatgt 2041 tccaaccttg cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg 2101 ctaacggtcg tcgtcctcta gcgctggtgc gtactcacag taagaaagca ctgatgcgct 2161 acgaggacgt ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat 2221 ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc 2281 cggtcgagga catccctgcg attgagcgtg aagaactccc gatgaaaccg gaggacatcg 2341 acatgaatcc tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg 2401 acaaggctcg caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt 2461 ttgctaacca taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg 2521 ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga 2581 aaggtaaacc aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg 2641 cgggtgtcga taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga 2701 acatcatggc ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc 2761 cgttctgctt ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct 2821 ataactgctc ccttccgctg gcgtttgacg ggtcttgctc cggcatccag cacttctccg 2881 cgatgctccg agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc 2941 aggacatcta cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca 3001 atgggaccga taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga 3061 aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca 3121 gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc 3181 aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca 3241 ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga 3301 cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg 3361 ctgaggtcaa agataagaaa actggagaga ttcttcgcaa gcgttgcgct gtgcattggg 3421 taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga 3481 acctgatgtt cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg 3541 agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg 3601 gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg 3661 cactgattca cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag 3721 tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc 3781 agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag 3841 gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcgtaa taacgcatga 3901 gacg //