##gff-version 3
##sequence-region BBa_J119078 1 3904
BBa_J119078	.	mature_transcript_region	1	1069	.	+	0	ID=BBa_K199191;Name=BBa_K199191
BBa_J119078	.	mature_transcript_region	927	1069	.	+	0	ID=BBa_K199045;Name=BBa_K199045;Parent=BBa_K199191
BBa_J119078	.	sequence_feature	972	976	.	+	0	ID=annotation2011866;Name=5nt anti codon;Parent=BBa_K199045
BBa_J119078	.	mature_transcript_region	568	710	.	+	0	ID=BBa_K199014;Name=BBa_K199014;Parent=BBa_K199191
BBa_J119078	.	sequence_alteration	613	617	.	+	0	ID=annotation2007934;Name=5 nt anticodon;Parent=BBa_K199014
BBa_J119078	.	mature_transcript_region	209	351	.	+	0	ID=BBa_K199048;Name=BBa_K199048;Parent=BBa_K199191
BBa_J119078	.	sequence_feature	254	258	.	+	0	ID=annotation2011945;Name=5nt anti codon;Parent=BBa_K199048
BBa_J119078	.	promoter	360	559	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K199191
BBa_J119078	.	non_covalent_binding_site	448	485	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J119078	.	engineered_region	360	559	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J119078	.	non_covalent_binding_site	525	559	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J119078	.	start_codon	532	532	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J119078	.	promoter	496	501	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J119078	.	CDS	360	447	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J119078	.	promoter	520	525	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J119078	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K199191
BBa_J119078	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J119078	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J119078	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J119078	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J119078	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J119078	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J119078	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J119078	.	promoter	719	918	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K199191
BBa_J119078	.	non_covalent_binding_site	807	844	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J119078	.	engineered_region	719	918	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J119078	.	non_covalent_binding_site	884	918	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J119078	.	start_codon	891	891	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J119078	.	promoter	855	860	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J119078	.	CDS	719	806	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J119078	.	promoter	879	884	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J119078	.	CDS	1078	3904	.	+	0	ID=BBa_J119060;Name=BBa_J119060
BBa_J119078	.	promoter	1078	1133	.	+	0	ID=annotation2169877;Name=pLacI;Parent=BBa_J119060
BBa_J119078	.	ribosome_entry_site	1145	1159	.	+	0	ID=annotation2169878;Name=B0030;Parent=BBa_J119060
BBa_J119078	.	sequence_alteration	1192	1203	.	+	0	ID=annotation2169880;Name=22 nt frameshift mutation;Parent=BBa_J119060
BBa_J119078	.	CDS	1166	3887	.	+	0	ID=annotation2169879;Name=T7 RNAP;Parent=BBa_J119060
>BBa_J119078
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcgg
ctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgca
tatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccga
ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagct
cactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt
cacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgtcctaaaccggagtagggg
caactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagctt
actagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgcc
ggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgc
cactgcatatccttagcgaaagctaaggattttttttaagcttactagagcgttgacaccatcgaatggcgcaaaacct
ttcgcggtatggcatgatagcgcccggcgtctcggcgaattaaagaggagaaagtaggcatgaacacgagagaccacgc
gatcgcgccaccgctactctagcgctaccaggcgcgccaggtctctgctggaagacgtcacgattaacatcgctaagaa
cgacttctctgacatcgaactggctgctatcccgttcaacactctagctgaccattacggtgagcgtttagctcgcgaa
cagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctg
gtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactg
gtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgta
gcgtacatcaccattaagaccactctagcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaa
tcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgtcga
ggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaag
ggtctactcggtggcgaggcgtggtcctcgtggcataaggaggactctattcatgtaggagtacgctgcatcgagatgc
tcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaact
cgcacctgaatacgctgaggctatcgcaacccgtgcaggggcgctggctggcatctctccgatgttccaaccttgcgta
gttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctagcgctggtgcgta
ctcacagtaagaaagcactgatgcgctacgaggacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaa
caccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggac
atccctgcgattgagcgtgaagaactcccgatgaaaccggaggacatcgacatgaatcctgaggctctcaccgcgtgga
aacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagca
agccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtca
atgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggtt
actactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattga
ggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttc
tgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgt
ttgacgggtcttgctccggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgct
tcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaat
gggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaagg
cactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtc
caaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttc
actcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttg
aagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaaaactggagagattcttcg
caagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgc
ttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacaca
aacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcaca
cgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttc
aaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgacc
agttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtc
ggacttcgcgttcgcgtaataacgcatgagacg