BBa_J119083 1 BBa_J119083 RFP Node 2012-03-09T12:00:00Z 2015-08-31T04:08:27Z de novo This part is one of several similar parts designed in our lab to serve as "Nodes" in a Hamiltonian Path Problem experiment using Golden Gate Assembly. This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a "Gene of Interest", which in this case is a RFP reporter. false false _613_ 0 10387 9 Not in stock false This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a "Gene of Interest", which in this case is a RFP reporter. false David Carr annotation2170613 1 PstI cuts right range2170613 1 711 716 annotation2170608 1 BbsI cuts right range2170608 1 11 16 annotation2170610 1 RFP Node (2nd Codon) range2170610 1 22 699 annotation2170607 1 EcoRI cuts left range2170607 1 5 10 annotation2170611 1 Exit Word (RFP) range2170611 1 700 703 annotation2170609 1 BbsI word (Node Word) range2170609 1 18 21 annotation2170606 1 EcoRI Word range2170606 1 1 4 annotation2170612 1 BsaI cuts left range2170612 1 705 710 BBa_J119083_sequence 1 gaattcgaagacaaatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataactgctgagaccctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z