BBa_J119084 1 BBa_J119084 GFP Node for GGA/HPP 2.0 2012-03-09T12:00:00Z 2015-08-31T04:08:27Z de novo This part is one of several similar parts designed in our lab to serve as "Nodes" in a Hamiltonian Path Problem experiment using Golden Gate Assembly. This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a "Gene of Interest", which in this case is a GFP reporter. false false _613_ 0 10387 9 Not in stock false This part contains four Type II restriction enzymes (BsaI, BbsI, PstI, and EcoRI) cutting sights which flank a "Gene of Interest", which in this case is a GFP reporter. false David Carr annotation2170620 1 BsaI cuts left range2170620 1 744 749 annotation2170614 1 EcoRI Word range2170614 1 1 4 annotation2170621 1 PstI cuts right range2170621 1 750 755 annotation2170618 1 GFP Node (2nd Codon) range2170618 1 22 738 annotation2170616 1 BbsI cuts right range2170616 1 11 16 annotation2170615 1 EcoRI cuts left range2170615 1 5 10 annotation2170617 1 BbsI word (Node Word) range2170617 1 18 21 annotation2170619 1 Exit Word (GFP) range2170619 1 739 742 BBa_J119084_sequence 1 gaattcgaagacaaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagatcacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaaccctgagaccctgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z