BBa_J119088
type: ComponentDefinition
persistent identity: BBa J119088
display id: BBa_J119088
version: 1
was generated by: iGEM to SBOL conversion
description: pT7-RBS-GFP-tRNA (K191)
created: 2012-03-18T12:00:00Z
modified: 2015-08-31T04:08:27Z
mutable provenance: From existing parts.
owned by: james
owned by: myers
mutable description: pT7-RBS-GFP-tRNA (K191)
discontinued: false
dominant: true
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Michel Conn
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K199176
component: BBa_K199188
sequence annotation: BBa_K199176
sequence annotation: BBa_K199188
sequence: https://synbiohub.org/public/igem/BBa_J119088_sequence/1

BBa_K199176
type: Component
persistent identity: component2170717
display id: component2170717
version: 1
top level: BBa_J119088
definition: BBa_K199176
access: public

BBa_K199188
type: Component
persistent identity: component2170745
display id: component2170745
version: 1
top level: BBa_J119088
definition: BBa_K199188
access: public

BBa_K199176
type: SequenceAnnotation
persistent identity: annotation2170717
display id: annotation2170717
version: 1
top level: BBa_J119088
location: https://synbiohub.org/public/igem/BBa_J119088/annotation2170717/range2170717/1
component: BBa_K199176

https://synbiohub.org/public/igem/BBa_J119088/annotation2170717/range2170717/1
type: Range
persistent identity: range2170717
display id: range2170717
version: 1
top level: BBa_J119088
direction: direction/reverse
start: 1
end: 772
orientation: inline

BBa_K199188
type: SequenceAnnotation
persistent identity: annotation2170745
display id: annotation2170745
version: 1
top level: BBa_J119088
location: https://synbiohub.org/public/igem/BBa_J119088/annotation2170745/range2170745/1
component: BBa_K199188

https://synbiohub.org/public/igem/BBa_J119088/annotation2170745/range2170745/1
type: Range
persistent identity: range2170745
display id: range2170745
version: 1
top level: BBa_J119088
direction: direction/reverse
start: 781
end: 1849
orientation: inline

BBa_K199176
type: ComponentDefinition
persistent identity: BBa K199176
display id: BBa_K199176
version: 1
was generated by: iGEM to SBOL conversion
description: pT7+RBS+GFP
created: 2010-07-15T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: BioBricks
owned by: james
owned by: myers
mutable description: This part expresses GFP in the presence of T7 RNA polymerase.
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_E0040
component: BBa_B0030
component: BBa_R0085
sequence annotation: BBa_R0085
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_K199176_sequence/1

BBa_E0040
type: Component
persistent identity: component2074004
display id: component2074004
version: 1
top level: BBa_K199176
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component2074001
display id: component2074001
version: 1
top level: BBa_K199176
definition: BBa_B0030
access: public

BBa_R0085
type: Component
persistent identity: component2073999
display id: component2073999
version: 1
top level: BBa_K199176
definition: BBa_R0085
access: public

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation2073999
display id: annotation2073999
version: 1
top level: BBa_K199176
location: https://synbiohub.org/public/igem/BBa_K199176/annotation2073999/range2073999/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_K199176/annotation2073999/range2073999/1
type: Range
persistent identity: range2073999
display id: range2073999
version: 1
top level: BBa_K199176
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2074001
display id: annotation2074001
version: 1
top level: BBa_K199176
location: https://synbiohub.org/public/igem/BBa_K199176/annotation2074001/range2074001/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K199176/annotation2074001/range2074001/1
type: Range
persistent identity: range2074001
display id: range2074001
version: 1
top level: BBa_K199176
direction: direction/forward
start: 32
end: 46
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2074004
display id: annotation2074004
version: 1
top level: BBa_K199176
location: https://synbiohub.org/public/igem/BBa_K199176/annotation2074004/range2074004/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K199176/annotation2074004/range2074004/1
type: Range
persistent identity: range2074004
display id: range2074004
version: 1
top level: BBa_K199176
direction: direction/reverse
start: 53
end: 772
orientation: inline

BBa_K199188
type: ComponentDefinition
persistent identity: BBa K199188
display id: BBa_K199188
version: 1
was generated by: iGEM to SBOL conversion
description: pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACU
created: 2010-07-20T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: BioBricks
owned by: james
owned by: myers
mutable description: pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACU
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_R0010
component: BBa_K199048
component: BBa_K199014
component: BBa_K199046
sequence annotation: BBa_R0010
sequence annotation: BBa_K199048
sequence annotation: BBa_K199014
sequence annotation: BBa_K199046
sequence: https://synbiohub.org/public/igem/BBa_K199188_sequence/1

BBa_R0010
type: Component
persistent identity: component2074946
display id: component2074946
version: 1
top level: BBa_K199188
definition: LacI
access: public

BBa_R0010
type: Component
persistent identity: component2074955
display id: component2074955
version: 1
top level: BBa_K199188
definition: LacI
access: public

BBa_K199048
type: Component
persistent identity: component2074954
display id: component2074954
version: 1
top level: BBa_K199188
definition: BBa_K199048
access: public

BBa_R0010
type: Component
persistent identity: component2074964
display id: component2074964
version: 1
top level: BBa_K199188
definition: LacI
access: public

BBa_K199014
type: Component
persistent identity: component2074963
display id: component2074963
version: 1
top level: BBa_K199188
definition: BBa_K199014
access: public

BBa_K199046
type: Component
persistent identity: component2074972
display id: component2074972
version: 1
top level: BBa_K199188
definition: BBa_K199046
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2074946
display id: annotation2074946
version: 1
top level: BBa_K199188
location: https://synbiohub.org/public/igem/BBa_K199188/annotation2074946/range2074946/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199188/annotation2074946/range2074946/1
type: Range
persistent identity: range2074946
display id: range2074946
version: 1
top level: BBa_K199188
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K199048
type: SequenceAnnotation
persistent identity: annotation2074954
display id: annotation2074954
version: 1
top level: BBa_K199188
location: https://synbiohub.org/public/igem/BBa_K199188/annotation2074954/range2074954/1
component: BBa_K199048

https://synbiohub.org/public/igem/BBa_K199188/annotation2074954/range2074954/1
type: Range
persistent identity: range2074954
display id: range2074954
version: 1
top level: BBa_K199188
direction: direction/reverse
start: 209
end: 351
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2074955
display id: annotation2074955
version: 1
top level: BBa_K199188
location: https://synbiohub.org/public/igem/BBa_K199188/annotation2074955/range2074955/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199188/annotation2074955/range2074955/1
type: Range
persistent identity: range2074955
display id: range2074955
version: 1
top level: BBa_K199188
direction: direction/forward
start: 360
end: 559
orientation: inline

BBa_K199014
type: SequenceAnnotation
persistent identity: annotation2074963
display id: annotation2074963
version: 1
top level: BBa_K199188
location: https://synbiohub.org/public/igem/BBa_K199188/annotation2074963/range2074963/1
component: BBa_K199014

https://synbiohub.org/public/igem/BBa_K199188/annotation2074963/range2074963/1
type: Range
persistent identity: range2074963
display id: range2074963
version: 1
top level: BBa_K199188
direction: direction/reverse
start: 568
end: 710
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2074964
display id: annotation2074964
version: 1
top level: BBa_K199188
location: https://synbiohub.org/public/igem/BBa_K199188/annotation2074964/range2074964/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199188/annotation2074964/range2074964/1
type: Range
persistent identity: range2074964
display id: range2074964
version: 1
top level: BBa_K199188
direction: direction/forward
start: 719
end: 918
orientation: inline

BBa_K199046
type: SequenceAnnotation
persistent identity: annotation2074972
display id: annotation2074972
version: 1
top level: BBa_K199188
location: https://synbiohub.org/public/igem/BBa_K199188/annotation2074972/range2074972/1
component: BBa_K199046

https://synbiohub.org/public/igem/BBa_K199188/annotation2074972/range2074972/1
type: Range
persistent identity: range2074972
display id: range2074972
version: 1
top level: BBa_K199188
direction: direction/reverse
start: 927
end: 1069
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

BBa_K199014
type: ComponentDefinition
persistent identity: BBa K199014
display id: BBa_K199014
version: 1
was generated by: iGEM to SBOL conversion
description: AGGAC tRNA Suppressor (Produces Serine)
created: 2009-07-01T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: Synthesized from oligonucleotides and sequence verified.
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' CUGUCCUAA 3'so that the anticodon GUCCA could be used to decode a frameshift mutation of AGGAC in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: William Vernon
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5 nt anticodon
sequence: https://synbiohub.org/public/igem/BBa_K199014_sequence/1

5 nt anticodon
type: SequenceAnnotation
persistent identity: annotation2007934
display id: annotation2007934
version: 1
top level: BBa_K199014
location: https://synbiohub.org/public/igem/BBa_K199014/annotation2007934/range2007934/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K199014/annotation2007934/range2007934/1
type: Range
persistent identity: range2007934
display id: range2007934
version: 1
top level: BBa_K199014
direction: direction/forward
start: 46
end: 50
orientation: inline

BBa_K199046
type: ComponentDefinition
persistent identity: BBa K199046
display id: BBa_K199046
version: 1
was generated by: iGEM to SBOL conversion
description: CUACU tRNA Suppressor (Produces Serine)
created: 2009-07-19T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: Oligo assembly
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' UUAGUAGAU 3'so that the anticodon AGUAG could be used to decode a frameshift mutation of CUACU in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: William Vernon
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: 5nt anti codon
sequence: https://synbiohub.org/public/igem/BBa_K199046_sequence/1

5nt anti codon
type: SequenceAnnotation
persistent identity: annotation2011943
display id: annotation2011943
version: 1
top level: BBa_K199046
location: https://synbiohub.org/public/igem/BBa_K199046/annotation2011943/range2011943/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199046/annotation2011943/range2011943/1
type: Range
persistent identity: range2011943
display id: range2011943
version: 1
top level: BBa_K199046
direction: direction/forward
start: 46
end: 50
orientation: inline

BBa_K199048
type: ComponentDefinition
persistent identity: BBa K199048
display id: BBa_K199048
version: 1
was generated by: iGEM to SBOL conversion
description: CCACC tRNA Suppressor (Produces Serine)
created: 2009-07-19T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: Oligo assembly
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' UUGGUGGAA 3'so that the anticodon GGUGG could be used to decode a frameshift mutation of CCACC in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: William Vernon
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: 5nt anti codon
sequence: https://synbiohub.org/public/igem/BBa_K199048_sequence/1

5nt anti codon
type: SequenceAnnotation
persistent identity: annotation2011945
display id: annotation2011945
version: 1
top level: BBa_K199048
location: https://synbiohub.org/public/igem/BBa_K199048/annotation2011945/range2011945/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199048/annotation2011945/range2011945/1
type: Range
persistent identity: range2011945
display id: range2011945
version: 1
top level: BBa_K199048
direction: direction/forward
start: 46
end: 50
orientation: inline

https://synbiohub.org/public/igem/BBa_J119088_sequence/1
type: Sequence
persistent identity: BBa J119088 sequence
display id: BBa_J119088_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: taatacgactcactatagggagatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgtcctaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttagtagataccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199176_sequence/1
type: Sequence
persistent identity: BBa K199176 sequence
display id: BBa_K199176_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199188_sequence/1
type: Sequence
persistent identity: BBa K199188 sequence
display id: BBa_K199188_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgtcctaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttagtagataccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199014_sequence/1
type: Sequence
persistent identity: BBa K199014 sequence
display id: BBa_K199014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtctgtcctaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199046_sequence/1
type: Sequence
persistent identity: BBa K199046 sequence
display id: BBa_K199046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtttagtagataccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199048_sequence/1
type: Sequence
persistent identity: BBa K199048 sequence
display id: BBa_K199048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z