LOCUS       BBa_J119096             1832 bp    DNA     linear       22-Dec-2024
DEFINITION  pT7-RBS-RFP-pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACU
ACCESSION   BBa_J119096
VERSION     BBa_J119096.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..724
                     /label=mrfp1
     misc_feature    731..755
                     /label=Help:Barcodes
     CDS             50..755
                     /label=BBa_E1010
     misc_feature    1..755
                     /label=BBa_K199118
     CDS             764..851
                     /label=end of LacI coding region (inactive)
     misc_feature    764..963
                     /label=BBa_R0010
     misc_feature    852..889
                     /label=CAP binding site
     promoter        900..905
                     /label=-35
     promoter        924..929
                     /label=-10
     misc_feature    929..963
                     /label=LacI binding site
     misc_feature    936..936
                     /label=start
     promoter        764..963
                     /label=BBa_R0010
     misc_feature    1017..1021
                     /label=5nt anti codon
     misc_feature    972..1114
                     /label=BBa_K199048
     CDS             1123..1210
                     /label=end of LacI coding region (inactive)
     misc_feature    1123..1322
                     /label=BBa_R0010
     misc_feature    1211..1248
                     /label=CAP binding site
     promoter        1259..1264
                     /label=-35
     promoter        1283..1288
                     /label=-10
     misc_feature    1288..1322
                     /label=LacI binding site
     misc_feature    1295..1295
                     /label=start
     promoter        1123..1322
                     /label=BBa_R0010
     misc_feature    1376..1380
                     /label=5 nt anticodon
     misc_feature    1331..1473
                     /label=BBa_K199014
     CDS             1482..1569
                     /label=end of LacI coding region (inactive)
     misc_feature    1482..1681
                     /label=BBa_R0010
     misc_feature    1570..1607
                     /label=CAP binding site
     promoter        1618..1623
                     /label=-35
     promoter        1642..1647
                     /label=-10
     misc_feature    1647..1681
                     /label=LacI binding site
     misc_feature    1654..1654
                     /label=start
     promoter        1482..1681
                     /label=BBa_R0010
     misc_feature    1735..1739
                     /label=5nt anti codon
     misc_feature    1690..1832
                     /label=BBa_K199046
     misc_feature    764..1832
                     /label=BBa_K199188
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      541 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      601 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      661 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      721 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagcaatacg caaaccgcct
      781 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      841 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      901 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      961 acatactaga gggatccaat tcggagagat gccggagcgg ctgaacggac cggtttggtg
     1021 gaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc gccactgcat
     1081 atccttagcg aaagctaagg atttttttta agcttactag agcaatacgc aaaccgcctc
     1141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1201 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     1261 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     1321 catactagag ggatccaatt cggagagatg ccggagcggc tgaacggacc ggtctgtcct
     1381 aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg ccactgcata
     1441 tccttagcga aagctaagga ttttttttaa gcttactaga gcaatacgca aaccgcctct
     1501 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     1561 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1621 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1681 atactagagg gatccaattc ggagagatgc cggagcggct gaacggaccg gtttagtaga
     1741 taccggagta ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactgcatat
     1801 ccttagcgaa agctaaggat tttttttaag ct
//