BBa_J119097
type: ComponentDefinition
persistent identity: BBa J119097
display id: BBa_J119097
version: 1
was generated by: iGEM to SBOL conversion
description: pT7-RBS-RFP-pLac+tRNA CCACC+pLac+tRNA CUAGC+pLac+tRNA CUACC
created: 2012-04-23T11:00:00Z
modified: 2015-08-31T04:08:27Z
mutable provenance: Existing parts.
owned by: james
owned by: myers
mutable description: Promoter for T7 RNAP polymerase drives RFP expression. Three frameshift suppression tRNAs produced: CCACC+CUAGC+CUACC.
discontinued: false
dominant: false
experience: experience/None
group u list: _613_435_
m user id: 0
owner id: 606
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Jerrad Morton
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K199189
component: BBa_K199118
sequence annotation: BBa_K199118
sequence annotation: BBa_K199189
sequence: https://synbiohub.org/public/igem/BBa_J119097_sequence/1

BBa_K199189
type: Component
persistent identity: component2278140
display id: component2278140
version: 1
top level: BBa_J119097
definition: BBa_K199189
access: public

BBa_K199118
type: Component
persistent identity: component2278112
display id: component2278112
version: 1
top level: BBa_J119097
definition: BBa_K199118
access: public

BBa_K199118
type: SequenceAnnotation
persistent identity: annotation2278112
display id: annotation2278112
version: 1
top level: BBa_J119097
location: https://synbiohub.org/public/igem/BBa_J119097/annotation2278112/range2278112/1
component: BBa_K199118

https://synbiohub.org/public/igem/BBa_J119097/annotation2278112/range2278112/1
type: Range
persistent identity: range2278112
display id: range2278112
version: 1
top level: BBa_J119097
direction: direction/reverse
start: 1
end: 755
orientation: inline

BBa_K199189
type: SequenceAnnotation
persistent identity: annotation2278140
display id: annotation2278140
version: 1
top level: BBa_J119097
location: https://synbiohub.org/public/igem/BBa_J119097/annotation2278140/range2278140/1
component: BBa_K199189

https://synbiohub.org/public/igem/BBa_J119097/annotation2278140/range2278140/1
type: Range
persistent identity: range2278140
display id: range2278140
version: 1
top level: BBa_J119097
direction: direction/reverse
start: 764
end: 1832
orientation: inline

BBa_K199118
type: ComponentDefinition
persistent identity: BBa K199118
display id: BBa_K199118
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter + RBS + RFP
created: 2010-03-02T12:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: Biobrick parts.
owned by: james
owned by: myers
mutable description: T7 Promoter (R0085) + RBS (B0034) + RFP (E1010)
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5118
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Clifton Davis
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0034
component: BBa_R0085
component: BBa_E1010
sequence annotation: BBa_R0085
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K199118_sequence/1

BBa_B0034
type: Component
persistent identity: component2247070
display id: component2247070
version: 1
top level: BBa_K199118
definition: BBa_B0034
access: public

BBa_R0085
type: Component
persistent identity: component2247068
display id: component2247068
version: 1
top level: BBa_K199118
definition: BBa_R0085
access: public

BBa_E1010
type: Component
persistent identity: component2247073
display id: component2247073
version: 1
top level: BBa_K199118
definition: mRFP1
access: public

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation2247068
display id: annotation2247068
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247068/range2247068/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_K199118/annotation2247068/range2247068/1
type: Range
persistent identity: range2247068
display id: range2247068
version: 1
top level: BBa_K199118
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2247070
display id: annotation2247070
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247070/range2247070/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K199118/annotation2247070/range2247070/1
type: Range
persistent identity: range2247070
display id: range2247070
version: 1
top level: BBa_K199118
direction: direction/reverse
start: 32
end: 43
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2247073
display id: annotation2247073
version: 1
top level: BBa_K199118
location: https://synbiohub.org/public/igem/BBa_K199118/annotation2247073/range2247073/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K199118/annotation2247073/range2247073/1
type: Range
persistent identity: range2247073
display id: range2247073
version: 1
top level: BBa_K199118
direction: direction/reverse
start: 50
end: 755
orientation: inline

BBa_K199189
type: ComponentDefinition
persistent identity: BBa K199189
display id: BBa_K199189
version: 1
was generated by: iGEM to SBOL conversion
description: pLac+tRNA CCACC+pLac+tRNA CUAGC+pLac+tRNA CUACC
created: 2010-07-20T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: BioBricks
owned by: james
owned by: myers
mutable description: pLac+tRNA CCACC+pLac+tRNA CUAGC+pLac+tRNA CUACC
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5111
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: SO:0000834
role: partType/RNA
component: BBa_R0010
component: BBa_K199048
component: BBa_K199016
component: BBa_K199045
sequence annotation: BBa_R0010
sequence annotation: BBa_K199048
sequence annotation: BBa_K199016
sequence annotation: BBa_K199045
sequence: https://synbiohub.org/public/igem/BBa_K199189_sequence/1

BBa_R0010
type: Component
persistent identity: component2074973
display id: component2074973
version: 1
top level: BBa_K199189
definition: LacI
access: public

BBa_R0010
type: Component
persistent identity: component2074982
display id: component2074982
version: 1
top level: BBa_K199189
definition: LacI
access: public

BBa_K199048
type: Component
persistent identity: component2074981
display id: component2074981
version: 1
top level: BBa_K199189
definition: BBa_K199048
access: public

BBa_R0010
type: Component
persistent identity: component2074991
display id: component2074991
version: 1
top level: BBa_K199189
definition: LacI
access: public

BBa_K199016
type: Component
persistent identity: component2074990
display id: component2074990
version: 1
top level: BBa_K199189
definition: BBa_K199016
access: public

BBa_K199045
type: Component
persistent identity: component2074999
display id: component2074999
version: 1
top level: BBa_K199189
definition: BBa_K199045
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2074973
display id: annotation2074973
version: 1
top level: BBa_K199189
location: https://synbiohub.org/public/igem/BBa_K199189/annotation2074973/range2074973/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199189/annotation2074973/range2074973/1
type: Range
persistent identity: range2074973
display id: range2074973
version: 1
top level: BBa_K199189
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K199048
type: SequenceAnnotation
persistent identity: annotation2074981
display id: annotation2074981
version: 1
top level: BBa_K199189
location: https://synbiohub.org/public/igem/BBa_K199189/annotation2074981/range2074981/1
component: BBa_K199048

https://synbiohub.org/public/igem/BBa_K199189/annotation2074981/range2074981/1
type: Range
persistent identity: range2074981
display id: range2074981
version: 1
top level: BBa_K199189
direction: direction/reverse
start: 209
end: 351
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2074982
display id: annotation2074982
version: 1
top level: BBa_K199189
location: https://synbiohub.org/public/igem/BBa_K199189/annotation2074982/range2074982/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199189/annotation2074982/range2074982/1
type: Range
persistent identity: range2074982
display id: range2074982
version: 1
top level: BBa_K199189
direction: direction/forward
start: 360
end: 559
orientation: inline

BBa_K199016
type: SequenceAnnotation
persistent identity: annotation2074990
display id: annotation2074990
version: 1
top level: BBa_K199189
location: https://synbiohub.org/public/igem/BBa_K199189/annotation2074990/range2074990/1
component: BBa_K199016

https://synbiohub.org/public/igem/BBa_K199189/annotation2074990/range2074990/1
type: Range
persistent identity: range2074990
display id: range2074990
version: 1
top level: BBa_K199189
direction: direction/reverse
start: 568
end: 710
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2074991
display id: annotation2074991
version: 1
top level: BBa_K199189
location: https://synbiohub.org/public/igem/BBa_K199189/annotation2074991/range2074991/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199189/annotation2074991/range2074991/1
type: Range
persistent identity: range2074991
display id: range2074991
version: 1
top level: BBa_K199189
direction: direction/forward
start: 719
end: 918
orientation: inline

BBa_K199045
type: SequenceAnnotation
persistent identity: annotation2074999
display id: annotation2074999
version: 1
top level: BBa_K199189
location: https://synbiohub.org/public/igem/BBa_K199189/annotation2074999/range2074999/1
component: BBa_K199045

https://synbiohub.org/public/igem/BBa_K199189/annotation2074999/range2074999/1
type: Range
persistent identity: range2074999
display id: range2074999
version: 1
top level: BBa_K199189
direction: direction/reverse
start: 927
end: 1069
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

BBa_K199016
type: ComponentDefinition
persistent identity: BBa K199016
display id: BBa_K199016
version: 1
was generated by: iGEM to SBOL conversion
description: CUAGC tRNA Suppressor (Produces Serine)
created: 2009-07-01T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: Oligo assembly
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' CUGCUAGAA 3'so that the anticodon GCUAG could be used to decode a frameshift mutation of CUAGC in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5118
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Clifton Davis
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5 nt anitcodon
sequence: https://synbiohub.org/public/igem/BBa_K199016_sequence/1

5 nt anitcodon
type: SequenceAnnotation
persistent identity: annotation2007936
display id: annotation2007936
version: 1
top level: BBa_K199016
location: https://synbiohub.org/public/igem/BBa_K199016/annotation2007936/range2007936/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K199016/annotation2007936/range2007936/1
type: Range
persistent identity: range2007936
display id: range2007936
version: 1
top level: BBa_K199016
direction: direction/forward
start: 46
end: 50
orientation: inline

BBa_K199045
type: ComponentDefinition
persistent identity: BBa K199045
display id: BBa_K199045
version: 1
was generated by: iGEM to SBOL conversion
description: CUACC tRNA Suppressor (Produces Serine)
created: 2009-07-19T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: Assembly from oligos
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' GUGGUAGAA 3'so that the anticodon GGUAG could be used to decode a frameshift mutation of CUACC in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: NO
star: false
creator: William Vernon
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5nt anti codon
sequence: https://synbiohub.org/public/igem/BBa_K199045_sequence/1

5nt anti codon
type: SequenceAnnotation
persistent identity: annotation2011866
display id: annotation2011866
version: 1
top level: BBa_K199045
location: https://synbiohub.org/public/igem/BBa_K199045/annotation2011866/range2011866/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K199045/annotation2011866/range2011866/1
type: Range
persistent identity: range2011866
display id: range2011866
version: 1
top level: BBa_K199045
direction: direction/forward
start: 46
end: 50
orientation: inline

BBa_K199048
type: ComponentDefinition
persistent identity: BBa K199048
display id: BBa_K199048
version: 1
was generated by: iGEM to SBOL conversion
description: CCACC tRNA Suppressor (Produces Serine)
created: 2009-07-19T11:00:00Z
modified: 2015-05-08T01:11:19Z
mutable provenance: Oligo assembly
owned by: james
owned by: myers
mutable description: A serine tRNA was modified to have the anticodon loop 5' UUGGUGGAA 3'so that the anticodon GGUGG could be used to decode a frameshift mutation of CCACC in a mRNA.
discontinued: false
dominant: false
experience: experience/None
group u list: _295_
m user id: 0
owner id: 5115
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: no
star: false
creator: William Vernon
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: 5nt anti codon
sequence: https://synbiohub.org/public/igem/BBa_K199048_sequence/1

5nt anti codon
type: SequenceAnnotation
persistent identity: annotation2011945
display id: annotation2011945
version: 1
top level: BBa_K199048
location: https://synbiohub.org/public/igem/BBa_K199048/annotation2011945/range2011945/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K199048/annotation2011945/range2011945/1
type: Range
persistent identity: range2011945
display id: range2011945
version: 1
top level: BBa_K199048
direction: direction/forward
start: 46
end: 50
orientation: inline

https://synbiohub.org/public/igem/BBa_J119097_sequence/1
type: Sequence
persistent identity: BBa J119097 sequence
display id: BBa_J119097_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgctagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199118_sequence/1
type: Sequence
persistent identity: BBa K199118 sequence
display id: BBa_K199118_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199189_sequence/1
type: Sequence
persistent identity: BBa K199189 sequence
display id: BBa_K199189_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgctagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcggagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199016_sequence/1
type: Sequence
persistent identity: BBa K199016 sequence
display id: BBa_K199016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtctgctagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199045_sequence/1
type: Sequence
persistent identity: BBa K199045 sequence
display id: BBa_K199045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199048_sequence/1
type: Sequence
persistent identity: BBa K199048 sequence
display id: BBa_K199048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtttggtggaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z