LOCUS BBa_J119098 1832 bp DNA linear 25-Nov-2024 DEFINITION pT7-RBS-RFP-pLac+tRNA CCACC+pLac+tRNA CUAGC+pLac+tRNA CUACU ACCESSION BBa_J119098 VERSION BBa_J119098.1 FEATURES Location/Qualifiers misc_feature 18..18 /label=Transcription Start Site misc_feature 1..11 /label=Polymerase Binding Region misc_feature 12..23 /label=Initiation Region promoter 1..23 /label=BBa_R0085 misc_feature 36..39 /label=conserved RBS 32..43 /label=BBa_B0034 CDS 50..724 /label=mrfp1 misc_feature 731..755 /label=Help:Barcodes CDS 50..755 /label=BBa_E1010 misc_feature 1..755 /label=BBa_K199118 CDS 764..851 /label=end of LacI coding region (inactive) misc_feature 764..963 /label=BBa_R0010 misc_feature 852..889 /label=CAP binding site promoter 900..905 /label=-35 promoter 924..929 /label=-10 misc_feature 929..963 /label=LacI binding site misc_feature 936..936 /label=start promoter 764..963 /label=BBa_R0010 misc_feature 1017..1021 /label=5nt anti codon misc_feature 972..1114 /label=BBa_K199048 CDS 1123..1210 /label=end of LacI coding region (inactive) misc_feature 1123..1322 /label=BBa_R0010 misc_feature 1211..1248 /label=CAP binding site promoter 1259..1264 /label=-35 promoter 1283..1288 /label=-10 misc_feature 1288..1322 /label=LacI binding site misc_feature 1295..1295 /label=start promoter 1123..1322 /label=BBa_R0010 misc_feature 1376..1380 /label=5 nt anitcodon misc_feature 1331..1473 /label=BBa_K199016 CDS 1482..1569 /label=end of LacI coding region (inactive) misc_feature 1482..1681 /label=BBa_R0010 misc_feature 1570..1607 /label=CAP binding site promoter 1618..1623 /label=-35 promoter 1642..1647 /label=-10 misc_feature 1647..1681 /label=LacI binding site misc_feature 1654..1654 /label=start promoter 1482..1681 /label=BBa_R0010 misc_feature 1735..1739 /label=5nt anti codon misc_feature 1690..1832 /label=BBa_K199046 misc_feature 764..1832 /label=BBa_K199190 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc 61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg 121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc 181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca 241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa 301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt 361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact 421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga 481 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg 541 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa 601 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca 661 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg 721 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagcaatacg caaaccgcct 781 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 841 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 901 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 961 acatactaga gggatccaat tcggagagat gccggagcgg ctgaacggac cggtttggtg 1021 gaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc gccactgcat 1081 atccttagcg aaagctaagg atttttttta agcttactag agcaatacgc aaaccgcctc 1141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 1201 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 1261 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 1321 catactagag ggatccaatt cggagagatg ccggagcggc tgaacggacc ggtctgctag 1381 aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg ccactgcata 1441 tccttagcga aagctaagga ttttttttaa gcttactaga gcaatacgca aaccgcctct 1501 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 1561 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 1621 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 1681 atactagagg gatccaattc ggagagatgc cggagcggct gaacggaccg gtttagtaga 1741 taccggagta ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactgcatat 1801 ccttagcgaa agctaaggat tttttttaag ct //